紫外分光光度法测定富硒麦芽中微量元素硒的含量  

Determination of trace element selenium in selenium-rich malt with ultraviolet spectrophotometric method

作　　者：李丽彩[1] 王留成[1] 程相林[1] 赵建宏[1] 王建设[1] 

机构地区：[1]郑州大学化工与能源学院,河南郑州450001

出　　处：《应用化工》2016年第4期771-774,共4页Applied Chemical Industry

摘　　要：采用紫外分光光度法,以邻苯二胺作显色剂,波长334 nm,测定富硒麦芽中微量元素硒的含量。结果表明,线性方程为A=0.207 78c＋0.003 69,相关系数r=0.999 86;平均加标回收率为98.04%,RSD=4.4%;方法的精密度（RSD=2.7%）、重现性（RSD=1.4%）和稳定性（RSD=0.80%）均较好;富硒麦芽中硒的含量为0.6~42.1μg/g。该方法具有操作简单,测定快速,灵敏度高,毒性较小等优点,适用于测定麦芽中微量硒的含量。The content of Se in selenium-rich malt is determined with ultraviolet spectrophotometry with ophenylendiamine as the chromogenic agent under 334 nm detection wavelength. The result showed that the regression equation is A = 0. 207 78 c ＋ 0. 003 69,the correlation coefficient is 0. 999 86; The average recovery rate is 98. 04% and the RSD is 4. 4%; The precision（ RSD = 2. 7%）,reproducibility（ RSD = 1. 4%）and stability（ RSD = 0. 80%） of the method are good; The content of selenium in Se-enriched malt is 0. 6~ 42. 1 μg / g. This method has the advantage of simple operation,fast measurement,high sensitivity,a smaller toxicity and so on,especially applicable for the determination of trace element selenium in malt.

关 键 词：富硒麦芽 硒 含量 紫外分光光度法 

分 类 号：O657.32[理学—分析化学]