恩施州渔塘坝硒矿区天然高富硒微生物的筛选与鉴定  

Screening and Identification of Natural Selenium Super-Enriched Microorganisms in Enshi Yutangba′s Selenium Ore Area

作　　者：帅超群[1,2,3] 向极钎 谈弋[1] 杨永康 殷红清 覃大吉 陈菲菲 

机构地区：[1]武汉大学药学院,湖北武汉430072 [2]恩施清江生物工程有限公司,湖北恩施445000 [3]恩施州硒应用技术与产品开发研究院,湖北恩施445000 [4]恩施州农科院药物与园艺研究所,湖北恩施445000

出　　处：《化学与生物工程》2016年第4期51-54,共4页Chemistry & Bioengineering

基　　金：湖北省科技支撑计划资助项目(2013BEC048)

摘　　要：采用富集培养及平板划线法分离恩施州渔塘坝硒矿区中天然高富硒微生物。通过在基础发酵培养基中添加不同浓度亚硒酸钠,筛选出一株高富硒微生物XS-1,该菌株培养物最高硒含量可达34 782.52×10-6。通过形态学观察及ITS序列分析鉴定该菌株为异常威克汉姆酵母(Wickerhamomyces anomalus),对硒具有极高的耐受及富集能力。The natural selenium super-enriched microorganisms in Enshi Yutangba′s selenium ore area were isolated by enrichment culture and streak plate methods.The selenium super-enriched microorganism XS-1was screened by adding different concentrations of sodium selenite into the fermentation medium,and the highest selenium content in the fermentation products was 34 782.52×10-6.The microorganism XS-1was identified to be Wickerhamomyces anomalus by morphological observation and ITS sequence analysis.This microorganism has high tolerance and enrichment capacity to selenium.

关 键 词：硒矿区 富硒微生物 筛选 鉴定 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]