大气压化学电离源-质谱法分析花生四烯酸油脂成分  

Analysis of component of arachidonic acid oil by atmospheric pressure chemical ionization-mass spectrometry

作　　者：陆姝欢 余超 柴莎莎 汪志明 李翔宇 

机构地区：[1]嘉必优生物工程(武汉)有限公司,武汉430073 [2]湖北省营养化学品生物合成工程技术研究中心,武汉430223

出　　处：《中国油脂》2016年第4期92-95,共4页China Oils and Fats

基　　金：国家高技术研究发展计划(863计划)项目资助(2014AA021703)

摘　　要：采用大气压化学电离源-质谱法（APCI-MS）对花生四烯酸（ARA）油脂成分进行定性分析。结果表明：在APCI正离子模式,高压放电针电流8μA,样品锥孔电压5 V,离子源温度130℃,APCI加热器温度500℃,脱溶剂气流量300 L/h,锥孔气流量100 L/h,质量扫描范围（m/z）100~1 000的质谱条件下,分析得出ARA油脂中含有167种甘油酯,其中有10种甘油一酯、55种甘油二酯及102种甘油三酯。此方法简单、快捷,不仅适用于油脂中的饱和脂肪酸甘油酯的定性分析,还可以用于不饱和脂肪酸甘油酯的定性分析。Atmospheric pressure chemical ionization- mass spectrometry（ APCI- MS） was used for qualitative analysis of arachidonic acid（ ARA） oil. The results showed that under the conditions of APCI positive ion mode,high- pressure discharge electric current 8 μA,sample cone voltage 5 V,ion source temperature 130 ℃,APCI vaporizer temperature 500 ℃,flow of desolvation gas 300 L / h,flow of cone gas 100 L / h and scanning range（ m / z） of mass 100- 1 000,167 kinds of glycerides were determined,in which there were ten kinds of monoglycerides,55 kinds of diacylglycerols and 102 kinds of triglycerides. The method had the items of simpleness and quickness,which was not only suitable for qualitative analysis of saturated fatty acid glycerides,but also suitable for qualitative analysis of unsaturated fatty acid glycerides.

关 键 词：大气压化学电离源-质谱法(APCI-MS) 花生四烯酸(ARA)油脂 定性分析 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]