结直肠癌中CD44和上皮细胞黏附分子相关基因与通路的生物信息学研究  

Bioinformatics research of CD44 and epithelial cell adhesion molecule related genes and pathways in colorectal cancer

作　　者：马敏星 周瑞[1] 张嘉刚 马洪卫[2] 陶文惠[1] 赵秋[1] 李瑾[1] 

机构地区：[1]武汉大学中南医院消化内科湖北省肠病医学临床研究中心,430071 [2]武汉大学中南医院乳腺甲状腺外科,430071 [3]青海省第五人民医院消化内科

出　　处：《中华消化杂志》2016年第3期182-187,共6页Chinese Journal of Digestion

摘　　要：目的寻找结肠癌干细胞表面标志物CD44和上皮细胞黏附分子（EpCAM）表达密切相关的基因及其参与的生物过程。方法通过生物信息学方法，利用基因表达精选集（GEO）数据库中结直肠癌基因芯片数据，结合R2平台，筛选出与CD44和EpCAM表达显著相关的基因，分析相关基因在不同性别、肿瘤家族史、是否饮酒、Dukes分期中的表达差异，并对比相关基因在其他肿瘤及正常人体组织中的表达情况。同时，利用基因本体及京都基因和基因组百科全书（KEGG）工具对与CD44和EpCAM表达显著相关基因进行基因本体和KEGG通路分析。利用R2平台内嵌统计学工具，采用单因素方差分析和连续校正的四格表卡方检验等进行统计学分析。结果在此数据集315份结直肠癌标本中均检测出CD44和EpCAM表达，分别筛选出与CD44和EpCAM表达显著相关基因888个和6316个。CD44与EpCAM共同正相关，且在全部315份样本中均有表达的5个基因的表达与不同性别、肿瘤家族史、是否饮酒、Dukes分期均无明显关系（P均〉0．05）。通过与其他肿瘤及正常人体组织中的表达情况进一步对比发现，溶质载体家族12成员2（SLC12A2）、中心粒1蛋白组中心粒蛋白B（POC1B）2个基因在结直肠癌中的表达较其他肿瘤明显增高（F=289．422、128．456，P均〈0．01），并在结直肠癌中的表达较正常人体组织明显增高（F=349．519、128．456，P均〈0．01）。对基因本体分析后发现，CD44和EpCAM表达均相关的基因共同涉及15条基因本体语义。对与CD44和EpCAM均相关的基因进行KEGG通路分析后发现，分别有7条和10条通路，差异均有统计学意义（P均〈0．01）。结论CD44和EpCAM在结直肠癌中普遍表达，与CD44和EpCAM表达相关的基因参与了多种肿瘤生物过程。Objective To investigate genes and involved biological processes closely associated with stem cell markers of colorectal cancer-epithelial cell adhesion molecule （EpCAM）＋ and CD44＋. Methods By the bioinformatics method, with microarray data of colorectal cancer from gene expression omnibus （GEO） database and R2 platform, the genes significantly related with CD44 and EpCAM expression were screened out. The differences in expression of related genes were analyzed on the basis of gender, family history of cancer, alcohol and Dukes stage. The expression of related genes in eolorectal cancer was compared with that of other tumors and healthy subjects. At same time, the pathways of the genes and Kyoto encyclopedia of genes and genomes （KEGG） of CD44 and EpCAM significantly related genes were analyzed with gene ontology （GO） and KEGG method. Single factor analysis of variance and Chi-square test of four-fold table with correction for continuity were used for statistical analysis by R2 platform embedded statistical tools. Results The expressions of CD44 and EpCAM were detected in all .315 colorectal cancer samples. A total of 888 and 6 316 genes were screened out which were significantly associated with CD44 and EpCAM expression. CD44 was positively correlated with EDCAM. There was no obvious correlation between the expression of five genes which expressed in all 315 tissues and gender family history of cancer, alcohol and Dukes stage （all P〉0. 05）. By further compared with the expression in other tumors and tissues, the expressions of two genes solute carrier family 12, member 2 （SLC12A2） and proteome of centriole 1 centriolar protein B （POC1B） in colorectal tumor were significantly higher than that in other tumors （F= 289. 422,128. 456, all P〈0.01）, and its expression in colorectal cancer was obviously higher than that in tissues of health subjects （F=349. 519,128. 456, all P〈O. 01）. GO analysis indicated there were 15 GO semantics related with both CD44 and EpCAM. The gene

关 键 词：CD44 EPCAM 结直肠肿瘤 肿瘤干细胞 生物信息学 基因芯片 

分 类 号：R735.34[医药卫生—肿瘤]