一种灵敏简便的酶联免疫吸附测定法检测胃癌血清MG7抗原  

A sensitive and convenient enzyme-linked immunosorbent assay method in serum MG7 antigen detection in gastric cancer

作　　者：靳斌[1] 王新[2] 靳雁[2] 陈峥[2] 吴开春[2] 樊代明[2] 

机构地区：[1]西安市第一医院消化内科,710002 [2]第四军医大学西京消化病医院国家肿瘤生物学重点实验室

出　　处：《中华消化杂志》2016年第3期188-191,共4页Chinese Journal of Digestion

基　　金：国家重点基础研究发展计划（973计划）（2004CB518702）

摘　　要：目的为寻求一种高敏感性和高特异性的胃癌早期诊断方法，应用ELISA法检测胃癌患者的血清MG7抗原。方法用新建的ELISA法检测116例胃癌术前患者、63例胃癌术后患者、41例胃癌前病变患者、37例胃癌前疾病患者、50名健康者和281例其他肿瘤患者的血清MG7抗原。胃癌、胃癌前病变和胃癌前疾病的组织标本行MG7抗原免疫组织化学检测。采用卡方检验比较两种检测方法的阳性率。结果ELISA法检测MG7抗原的阳性率，在胃癌术前为83．6％（97／116），肺癌为45．2％（28／62），直肠癌为45．6％（20／44）、结肠癌为17．6％（12／68）、乳腺癌为14．2％（6／42）、胃癌术后为47．6％（30／63）、胃癌前病变为19．5％（8／41）、胃癌前疾病为5．4％（2／37）、健康人群为0。该ELISA法的敏感度（83．6％）与免疫组织化学法（94．0％）相似（P〉0．05）。EI。ISA法的假阳性率（12．8％）低于免疫组织化学法（51．3％）（X^2=26．491，P〈0．01）。不同临床分期胃癌MG7抗原的表达差异均有统计学意义（X^2=15．564，P〈0．01）。结论新建EI。ISA法可能成为胃癌高危人群非侵入的筛选方法。Objective To explore a highly sensitive and highly specific method to detect the serum MG7 antigen （Ag） level for early gastric cancer diagnosis. Methods The serum MG7-Ag level was detected by enzymeqinked immunosorbent assay （ELISA） method in 116 preoperative gastric cancer patients, 63 postoperative gastric cancer patients, 41 patients with precancerous lesion, 37 patients with precancerous diseases, 50 healthy individuals and 281 patients with other cancers. Meanwhile, the expression of MG7-Ag was also examined with immunohistochemistry in patients with gastric cancer or precancerous lesion. Chi square test was used for comparing positive rates of the two detection methods. Results The positive rate of MG7 Ag determined by ELISA was 83.6%（97/116） of preoperative gastric cancer patients, 45.2%（28/62） of lung cancer patients, 45.5%（20/44） of rectal cancer patients, 17.6%（12/68） of colonic cancer patients, 14. 2% （6/42） of breast cancer patients, 47. 6% （30/63） of postoperative gastric cancer patients, 19.5 % （8/41） of patients with precancerous lesions, 5.4% （2/37） of patients with precancerous diseases and 0 of healthy individuals. The sensitivity of ELISA （83.6 %） was similar with that of immunohistochemistry （94. 0%） （P）〉0. 05）. However, the false positive rate of ELISA （12.8%） was lower than that ofimmunohistochemistry（51.3%）（X2=26, 491,P〈0. 01）. There was statistically significant difference in MG7-Ag expression in gastric cancer with different clinical stages （X^2 = 15. 564,P〈0.01）. Conclusion This ELISA method might be a non-invasive screening method for population with high risk of gastric cancer.

关 键 词：酶联免疫吸附测定 胃肿瘤 MG7抗原 敏感性与特异性 

分 类 号：R735.2[医药卫生—肿瘤]