克氏原鳌虾产ESBLs大肠杆菌的分离鉴定与耐药研究  

Study on Antibiotic Resistance of Extended- Spectrum β- lactamases in Escherichia coli Isolated from Procambarus clarkii

作　　者：贾敏[1] 周伟[2] 韩一啸 梁冰[2] 梁津豪 纪雪[2] 孙洋[2] 刘军[2] 祝令伟[2] 陈萍[1] 郭学军[2] 

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118 [2]军事医学科学院军事兽医研究所,长春130122

出　　处：《中国兽药杂志》2016年第4期6-10,共5页Chinese Journal of Veterinary Drug

基　　金：国家"863"项目(2012AA022006);军队医药卫生课题(13CXZ024)

摘　　要：为了解克氏原螯虾产ESBLs大肠杆菌的耐药流行情况,采集来自山东、江苏、浙江、湖北和辽宁五省共计198份活体克氏原螯虾肠道样品,采用头孢噻肟抗性选择性培养基培养、PCR方法和BD PhoenixTM-100全自动微生物鉴定/药敏系统筛选、鉴定产ESBLs大肠杆菌并检测其药物敏感性。结果显示,从198份克氏原螯虾样品中共分离获得107株产ESBLs大肠杆菌,分离率为54.0%。所有分离菌株对青霉素类、一代头孢和部分三代头孢菌素均耐药;对四环素类、氟喹诺酮类、氯霉素类和磺胺类药物的耐药率均在60%以上(>65株);对氨基糖苷类药物耐药率较低;多重耐药(R≥3类)菌株所占比例为86.7%(96/107),且以5类(30%,31/107)和6类耐药(28%,30/107)为主。通过试验初步获得了克氏原螯虾产ESBLs大肠杆菌耐药水平的基础数据,为克氏原螯虾细菌耐药性防控和水产品公共卫生监测奠定基础。In order to explore the prevalence of ESBLs- producing E. coli and the status of antibiotic resistance in Procambarus clarkii,107 ESBLs- producing E. coli from 198 Procambarus clarkiis were collected from five provinces in China（ Shandong,Jiangsu,Zhejiang,Hubei,Liaoning）. The antimicrobial susceptibility and ESBLs phenotype of these stains were determined by cefotaxime- containing medium, PCR and BD PhoenixTM- 100 system. The results showed that ESBLs- producing E. coli ratio was 54%. All ESBLs-producing E. coli stains were resistant to penicillins,the first generation cephalosporins and part of the second generation cephalosporins. The average drug- resistance rate to tetracyclines,fluoroquinolones,chloramphenicols and sulfonamides was all above 60%（ 65 /107）. The resistance rate to aminoglycosides was lower. All strains were sensitive to the carbapenems. Among these ESBLs- producing E. coli strains,the percentage of multi- drug resistant（ R≥3classes） isolates was 86. 7%（ 96 /107）,isolates with resistance to five（ 30%,31 /107） or six（ 28%,30 /107） classes of drugs were predominated. This study obtained basic data about drug- resistance in ESBLs- producing E. coli. All the results were beneficial for monitoring public hygiene of aquatic product.

关 键 词：克氏原螯虾 产ESBLs大肠杆菌 多重耐药 

分 类 号：S852.61[农业科学—基础兽医学]