红景天苷对人乳腺癌MDA-MB-435细胞功能的作用  

Effect of Salidroside on the function of human breast cancer cell line MDA-MB-435

作　　者：龚舒 段承刚 陶忠桦 刘晓燕 傅俊江 甘淋[4] 

机构地区：[1]西南医科大学病理生理学教研室,四川泸州646000 [2]西南医科大学公共卫生学院,四川泸州646000 [3]西南医科大学医学基础研究中心,四川泸州646000 [4]西南医科大学生物化学教研室,四川泸州646000

出　　处：《泸州医学院学报》2016年第2期118-123,共6页Journal of Luzhou Medical College

基　　金：四川省科技厅应用基础研究计划项目(14JC0155)

摘　　要：目的:通过检测红景天苷对人乳腺癌MDA-MB-435细胞的增殖、凋亡、周期及运动侵袭的影响,探讨红景天苷抗人乳腺癌的作用。方法:体外培养MDA-MB-435细胞,CCK-8法筛查红景天苷对细胞的半数有效抑制浓度(IC50)。以不加红景天苷组为对照组,以IC50浓度的红景天苷组为实验组,在光镜下观察其形态改变,CCK-8法检测细胞增殖抑制率,流式细胞术检测细胞凋亡和细胞周期,Transwell小室模型观察细胞运动和侵袭能力的改变。结果:红景天苷对人乳腺癌MDA-MB-435细胞的IC50为4 mg/m L;光镜下见红景天苷组细胞呈现坏死和凋亡形态;CCK-8法显示红景天苷组在作用24 h、48 h和72 h三个时间点均能抑制细胞的生长增殖,抑制率均高于对照组,且具有显著的时间-效应依赖性(P<0.05);流式细胞术显示红景天苷组早期凋亡率为(3.31±0.21)%、晚期凋亡和坏死率为(28.80±2.15)%,高于对照组(1.26±0.13)%和(5.56±0.78)%,差异均具有统计学意义(P<0.05);细胞周期检测发现红景天苷组细胞较对照组在G0/G1期的比例增加,停滞在S期的比例减少,G2/M期的比例减少;运动实验结果显示,红景天苷作用72 h后,红景天苷组每个视野跨膜细胞数为(39.60±3.37)个,远低于对照组(142.20±12.99)个,差异具有统计学意义(P<0.01);侵袭实验结果示红景天苷组每个视野跨膜细胞数为(4.20±1.55)个,也低于对照组(13.00±3.09)个,差异具有统计学意义(P<0.05)。结论:4 mg/m L红景天苷作用人乳腺癌MDA-MB-435细胞,可抑制生长增殖、诱导细胞凋亡和坏死发生、阻滞细胞周期、有效抑制肿瘤细胞的运动和侵袭。Objective： To explore the effects and mechanisms of Salidroside on human breast cancer. Human breast cancer cell line MDA-MB-435 was treated with Salidroside（Sal）, and cell proliferation, apoptosis, cell migration and invasion were evaluated. Methods： The MDA-MB-435 cells were cultured in vitro. The half maximal inhibitory concentration（IC50） of Salidroside on MDA-MB-435 cells was determined by CCK-8 assay.MDA-MB-435 cells treated with IC50 of Sal were considered as the Sal group, and cells without Sal treatment were as the control group. Cell morphology was observed using light microscope, cell proliferation was detected by CCK-8 assay, apoptosis and cell cycle were evaluated by flow cytometry, and cell migration and invasion were studied using Transwell assay. Results： The IC50 of Sal on human breast cancer MDA-MB-435 cells was 4mg/m L. The cells treated with Sal showed the typical morphology of necrosis and apoptosis under lightmicroscropy. Using CCK-8 assay, cells proliferation was significantly inhibited at the IC50 of Sal at 24 h, 48 h and 72 h, relative to the control group（P 〈 0.05）, and the inhibition showed a time-dependent manner. Flow cytometry using AnnexinⅤ-FITC/PI apoptosis assay kit demonstrated that the percentage of early apoptotic cells was significantly increased（3.31 ± 0.21）% in the Sal group, compared to（1.26 ± 0.13）% in the control group（P〈 0.05）, and the late apoptosis and necrosis was also significantly induced（28.80 ± 2.15）% in the Sal group,compared to（5.56 ± 0.78）% in the control group（P 〈 0.01）. The results of FITC demonstrated that the ratio of G0/G1 was increased, while the cells at S phase and the ratio of G2/M were decreased compared to those of the control group. The cell migration and invasion assay revealed that the average number of invaded and migrated cells in the Sal group in migration assay（39.60 ± 3.37）, and in invasive assay（4.20 ± 1.55） were significantly decreased compared to the control group in migration

关 键 词：红景天苷 乳腺癌 增殖 凋亡 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]