高脂喂养SD大鼠体内花生四烯酸乙醇胺水平及Ⅰ型大麻素受体表达的变化  

Anandamide level and expression of cannabinoid receptor Ⅰ in SD rats fed with high fat diet

作　　者：陈华[1] 龙明[1] 封玉玲[1] 赖国旗[2] 李晶[3] 

机构地区：[1]重庆三峡医药高等专科学校,重庆404120 [2]重庆医科大学动物实验中心,重庆400016 [3]重庆三峡中心医院中心实验室,重庆404000

出　　处：《中国生物制品学杂志》2016年第4期374-377,共4页Chinese Journal of Biologicals

基　　金：重庆市基础与前沿研究计划项目(cstc2014jcyjA10049);重庆市高等学校青年骨干教师资助计划;重庆市卫生局医学科研项目(2012-1-096)

摘　　要：目的探讨高脂饮食诱导肥胖SD大鼠脂肪代谢紊乱对内源性大麻素系统(endocannabinoid system,ECS)的影响及其机制。方法建立高脂饮食诱导肥胖SD大鼠模型,每周称量大鼠体重;采用酶比色法测定大鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)和低密度脂蛋白(low-density lipoprotein cholesterol,LDL-C)水平;高效液相色谱法检测大鼠血浆中大麻内源性配体花生四烯酸乙醇胺(anandamide,AEA)的含量;Western blot法检测大鼠附睾脂肪组织中Ⅰ型大麻素受体(cannabinoid receptorⅠ,CB1)的表达。结果大鼠经高脂饮食持续喂养8周,体重均不同程度增加;血清中TC、TG、LDL-C和血浆中AEA水平均显著升高(P<0.05);附睾脂肪组织中CB1的表达量也显著增加(P<0.05)。结论通过高脂饲料成功诱导SD大鼠的TC、TG和LDL-C升高,使大鼠产生高脂血症;大鼠血清AEA水平和血浆中CB1表达的显著变化表明肥胖大鼠体内ECS在肥胖过程中发生了显著改变,本研究在一定程度上建立了ECS与肥胖之间的联系。Objective To investigate the effect of lipid metabolism disturbance induced by high fat diet in obese SD rats on endocannabinoid system（ECS） as well as the relevant mechanism. Methods High fat diet-induced obese SD rat model was established, and the model rats were weighed weekly. The total cholesterol（TC）, triglyceride（TG） and lowdensity lipoprotein cholesterol（LDL-C） levels in sera of rats were determined by enzyme colorimetric method, while the anandamide（AEA） content in plasma by HPLC, and the expression of cannabinoid receptor Ⅰ（CB1） in epididymal fat tissue by Western blot. Results The bodyweights of rats increased at different degrees after being fed with high fat diet for 8 weeks, while the TC, TG and LDL-C levels in sera, the AEA level in plasma as well as the expression level of CB1 in epididymal fat tissue increased significantly（P〈0. 05）. Conclusion The increases of TC, TG and LDL-C levels as well as hyperlipemia in SD rats were successfully induced. The remarkable changes of AEA level in sera and CB1 expression level in plasma indicated that the ECS of obese rats changed significantly. The relationship between ECS and adiposity was established at a certain degree in this study.

关 键 词：肥胖 花生四烯酸乙醇胺 Ⅰ型大麻素受体 

分 类 号：R-332[医药卫生]