新型优化多肽对口腔常见微生物的杀菌作用  

Bactericidal effect of a novel peptide on oral microorganisms

作　　者：营秀 孙应明[1] 陶睿[1] 张梦洁[1] 姜秀[1] 

机构地区：[1]安徽医科大学无锡临床学院口腔科,无锡医学硕士研究生214044

出　　处：《医学研究生学报》2016年第4期359-363,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81300877)

摘　　要：目的抗菌肽是近来口腔微生物研究的热点,文中研究新型抗菌多肽pm11对口腔常见微生物的抗菌作用及机制。方法通过测定pm11对口腔常见微生物的最小抑菌浓度（minimal inhibitory concentration,MIC）、最小杀菌浓度（minimal bactericide concentration,MBC）及杀菌动力学曲线的方法,分析其对游离细菌的作用,以及测定唾液环境下pm11的抑菌圈直径分析其在口腔环境中的杀菌能力,利用激光共聚焦显微镜观察对变异链球菌生物膜的杀菌作用,利用扫描电镜（SEM）观察细菌表面的结构变化。结果 pm11对8种微生物具有抗菌活性,且抗菌活性相差很大,MIC范围为2~256μg/m L,MBC波动范围为4μg/m L至〉256μg/m L。杀菌动力学结果显示致龋菌随着pm11作用时间的增加,存活细菌数显著下降。抑菌圈pm11的唾液组和超纯水组没有明显差异,CLSM结果表明生物膜内有死菌存在,SEM结果直观地看到pm11作用下的细菌细胞膜表面有明显的孔洞、细胞皱缩、细菌溶胀及细胞裂解或碎片。结论 pm11具有广谱的杀菌作用,具有潜在的实用价值和临床指导意义。Objective Antimicrobial peptides are the focus of recent research in oral microbiology. This study aimed to evaluate the activity of a novel antimicrobial peptide pm11 against oral microorganisms and its action mechanisms. Methods We analyzed the effect of pm11 on oral microorganisms and determined its antimicrobial activity in the saliva environment by measuring its minimal inhibitory concentration（ MIC）,minimal bactericide concentration（ MBC）,and bactericidal kinetics. We observed its bactericidal activity on the biofilms of streptococcus mutans by confocal laser scanning microscopy（ CLSM） and the structural changes in the bacterial membrane by scanning electron microscopy（ SEM）. Results The antimicrobial activity of pm11 varied greatly against different oral microorganisms,with its MIC values ranging from 2 μg / m L to〉 256 μg / m L and its MBC values from 2 μg / m L to 256μg /m L. The bactericidal kinetics showed a decreasing survival rate of bacteria with the lengthening of the intervention time. The inhibitory-zone diameters exhibited no significant indifference between the water solution and the sterile saliva solution. CLSM revealed an increased number of dead bacteria in the pm11-treated biofilms,while SEM manifested obvious changes in the shape of the bacteria membrane treated with pm11. Conclusion Our findings suggest that pm11 has a broad spectrum of antimicrobial activities on oral microorganisms and a potential value of clinical application.

关 键 词：菌肽 变异链球菌 血链球菌 龋病 

分 类 号：R781.5[医药卫生—口腔医学]