外源性硫化氢对大鼠肠缺血再灌注损伤后肠黏膜细胞凋亡的影响  

The Effects of Exogenous Hydrogen Sulfide on Intestinal Mucosal Cell Apoptosis after Intestinal Ischemia-reperfusion Injury in Rat

作　　者：李龙腾[1] 叶艳芳[1] 胡殿宇[1] 巩治华[1] 

机构地区：[1]郑州铁路职业技术学院,河南郑州450052

出　　处：《郑州铁路职业技术学院学报》2016年第1期48-50,共3页Journal of Zhengzhou Railway Vocational and Technical College

基　　金：郑州铁路职业技术学院重点科技课题(2013JKY004);河南省科技厅2015年河南省基础与前沿技术研究项目(152300410183)

摘　　要：目的研究外源性硫化氢(H2S)对肠缺血再灌注损伤(I/RI)后肠黏膜细胞凋亡的影响,为临床治疗肠I/RI的新药开发提供依据。方法以硫氢化钠(Na HS)作为硫化氢的外源性供体,将32只健康雄性Wistar大鼠分为四组:(A)正常对照组、(B)假手术组、(C)肠IR/I模型组、(D)肠IR/I+H2S(Na HS,14umol/kg)干预组,每组8只。通过HE染色观察小肠组织的形态学改变,并用流式细胞术检测肠黏膜细胞的凋亡率。结果肠IR/I+H2S组比肠IR/I组的肠黏膜损伤明显减轻,肠黏膜细胞的凋亡率也明显降低。结论在肠I/RI中,H2S能降低肠黏膜细胞的凋亡,起到保护肠黏膜的作用。Objective： To study the effects of exogenous hydrogen sulfide（ H2S） on intestinal mucosal cell apoptosis after intestinal ischemia- reperfusion injury（ I / RI）. Methods： Sodium hydrosulfide（ Na HS） was taken as a donor of H2 S. 32 Wistar rats were divided equally into 4 groups：（ A） Nomal group（ B） Sham operation group（ C） Intestinal I / RI model group（ D） Intestinal I / RI ＋ H2S（ Na HS,14 umol / kg） group. Intestinal tissue alteration was observed with HE assay. The apoptosis of intestinal mucosal cells was measured by flow cytometry. Results： Compared with I / RI group,the intestinal mucosal injury and cell apoptosis of I / RI ＋ H2 S group was significantly reduced.Conclusion： In the intestinal I / RI,H2 S can reduce the apoptosis of intestinal mucosal cells,and can also protect the intestinal mucosa.

关 键 词：硫化氢 缺血再灌注损伤 肠 细胞凋亡 

分 类 号：R364.1[医药卫生—病理学]