不同方案移植脐带间充质干细胞对硫代乙酰胺所致大鼠肝纤维化治疗效果的比较  

Comparison of Therapeutic Efficacy of Different Transplantation Projects in Treatment of Liver Fibrosis Rats Induced by Thioacetamide with Transplantation of Umbilical Cord Mesenchymal Stem Cells

作　　者：薛改[1,2] 陈则纱 刘建芳[1] 韩晓磊[1] 侯艳宁[1,2] 

机构地区：[1]解放军白求恩国际和平医院药剂科,石家庄050082 [2]河北医科大学研究生学院,石家庄050017

出　　处：《解放军医药杂志》2016年第4期12-17,21,共7页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：军队"十二五"重点课题(BWS11J002)

摘　　要：目的观察不同方案移植人脐带间充质干细胞(h UCMSCs)对硫代乙酰胺(TAA)所致大鼠肝纤维化的治疗效果。方法选取30只正常大鼠作为对照组,其余大鼠应用TAA建立肝纤维化模型,造模成功的60只随机分为模型组和治疗组,每组30只。对照组不作任何处理,模型组给予生理盐水,治疗组采用尾静脉注射的方式移植h UCMSCs。各组分别于单次治疗后1、2、4周和多次治疗后1周和1年收集大鼠血液和肝脏标本,观察体重,检测肝功能,观察肝脏病理变化及肝纤维化程度。结果模型组和治疗组大鼠体重均低于对照组,治疗组单次治疗后4周与多次治疗后1周体重高于模型组(P<0.05)。模型组在单次治疗1、2、4周和多次治疗1周后血氨(BAM)、胆红素(TBIL)和碱性磷酸酶(AKP)血清浓度高于对照组,白蛋白/球蛋白(ALB/GLB)低于对照组,多次治疗后1年BAM高于对照组(P<0.05,P<0.01)。治疗组单次治疗后1、2、4周及多次治疗后1周、1年BAM水平低于模型组(P<0.01);治疗组TBIL、AKP水平单次治疗后2、4周和多次治疗后1周低于模型组,ALB/GLB水平单次治疗后1、2、4周和多次治疗后1周高于模型组(P<0.05,P<0.01)。治疗组单次治疗后2、4周和多次治疗后1周和1年肝脏纤维化程度均轻于模型组,且多次治疗后1周和1年轻于单次治疗后2、4周(P<0.01)。结论 h UCMSCs移植可改善肝纤维化大鼠的肝功能和肝脏纤维化程度,治疗效果随着h UCMSCs移植次数的增多和恢复期的延长而提高。Objective To observe the therapeutic efficacy of different transplantation projects in treatment of liver fibrosis rats induced by thioacetamide（ TAA） with human umbilical cord mesenchymal stem cells（ h UCMSCs）.Methods A total of 30 normal rats were chosen as control group,and for the other rats,liver fibrosis models were established by TAA,and then 60 successful models were randomly divided into model group（ n = 30） and treatment group（ n = 30）. The control group was untreated. The model group was treated with physiological saline,while the treatment group was transplanted with h UCMSCs by vena caudalis injection. The blood and liver samples were collected 1 week（ 1T-1W）,2 weeks（ 1T-2W） and 4 weeks（ 1T-4W） after the single transplantation and 1 week（ 4T-1W） and 1 year（ 4T-1Y） after the multiple transplantation,and then the weight and liver functions were detected,and the liver pathological changes and hepatic fibrosis degrees were observed. Results The weight values in the model and treatment groups were lower than those in the control group,and the weigh values of 1T-4W and 4T-1W in the treatment group were higher than those in the model group（ P〈0. 05）,Compared with those of control group,in model group,the levels of blood ammonia（ BAM）,total bilirubin（ TBIL） and alkaline phosphatase（ AKP） in 1T-1W,1T-2W and 1T-4W after the single transplantation,and 4T-1W after the multiple transplantation were higher,and albumin/globin（ ALB/GLB） value was lower,and the BAM value in 4T-1Y after the multiple transplantation was higher（ P〈0. 05,P〈0. 01）. Compared with those of model group,in treatment group,the BAM values in 1T-1W,1T-2W and 1T-4W after the single transplantation,and 4T-1W and 4T-1Y after the multiple transplantation were lower（ P〈0. 01）; TBIL and AKP values in 1T-2W and 1T-4W after the single transplantation and 4T-1W after the multiple transplantation were lower,and ALB/GLB values in 1T-1W,1T-2W and 1T-4W after the single transplantation,a

关 键 词：脐带间充质干细胞 干细胞移植 肝纤维化 大鼠 Sprague-Dawley 

分 类 号：R575[医药卫生—消化系统] R-332[医药卫生—内科学]