肝细胞刺激因子对肝癌细胞和大鼠肝细胞增生作用的研究  

Effects of hepatic stimulating substance on the proliferation of hepatocytes and hepatic tumor cells

作　　者：吴国庆[1] 吴晓燕[1] 苗乃法[1] 冯永堂[1] 李在连[1] 

机构地区：[1]潍坊医学院免疫学研究所,山东潍坊261042

出　　处：《中国生化药物杂志》2002年第4期171-173,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：山东省自然科学基金项目 ( 92C0 82 5)

摘　　要：目的探讨肝细胞刺激因子 (HSS)对正常大鼠肝细胞和人肝癌细胞的作用特点。方法应用自制的HSS分别与正常大鼠肝脏细胞和人传代肝癌细胞共同培养不同时间 ,以促进肝细胞增生的IL 6为对照组 ,采用3 H TdR掺入法 ,观察HSS对大鼠肝细胞和人肝癌细胞分裂增生和细胞形态变化的影响。结果在培养早期HSS对正常大鼠肝细胞和人肝癌细胞均具有刺激增生的作用 ,随后HSS对这些细胞的增生具有明显的抑制作用 ,并伴随着细胞的死亡形态改变。结论HSS对人肝癌细胞和正常大鼠肝细胞的增生不仅有促进作用 ,而且具有较强的抑制作用 。PurposeTo investigate the effects of hepatic stimulating substance(HSS) on the proliferation of hepatocyte and hepatic tumor cells. MethodsRat normal hepatocytes or human hepatic tumor cells were cultured respectively with HSS. Cell proliferation was detected by the technique of 3 H TdR uptake, meanwhile morphologies of cultured cells were observed under microscope. IL 6, a well known hepatocyte proliferation inducer, was used as control.ResultsIn the early culture period(about 24 hours) HSS stimulated hepatocytes and hepatic tumor cells to proliferate.In the late culture period (about 48 hours) it significantly inhibited the cells proliferation and the cells showed the death appearance.ConclusionThe results indicate that HSS has bi directional effects on the proliferation of normal hepatocyte and hepatic tumor cells, stimulating or suppressing the cell proliferation at different phases of cell growth.

关 键 词：肝细胞刺激因子 肝细胞 肝癌细胞 增生作用 抑制作用 HSS 动物实验 

分 类 号：R735.7[医药卫生—肿瘤] R73－36[医药卫生—临床医学]