RNAi介导的WFDC2基因沉默抑制人卵巢浆液性上皮癌生长的体内实验  被引量:4

The role and mechanism of RNAi-mediated silencing of WFDC2 in the progression of human serous epithelial ovarian cancer in vivo study

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作  者:朱亚飞[1] 高国兰[2] 褚芳[1] 

机构地区:[1]赣南医学院第一附属医院妇产科,赣州341000 [2]中国医科大学航空总医院妇产科中国科学院北京转化医学研究院,北京100012

出  处:《现代妇产科进展》2016年第3期187-191,共5页Progress in Obstetrics and Gynecology

基  金:江西省教育厅基金(No:GJJ14675);赣南医学院科研项目(No:YB201301);赣南医学院重点科研项目(No:ZD201404)

摘  要:目的:利用前期已构建WFDC2基因稳定低表达的人浆液性卵巢癌细胞株SKOV3,构建裸鼠皮下移植瘤模型,观察肿瘤生长、转移特点的变化。方法:选择前期构建的稳定株进行体内实验。分组:未处理肿瘤细胞组(OEC组)、转染慢病毒空载体组(OEC-mock组)和转染WFDC2小干扰RNA组(OEC-WFDC2-si组)。组织块移植法建立二代卵巢癌荷瘤裸鼠模型。绘制各组的肿瘤生长曲线,实验终点时比较各组肿瘤的体积和重量,免疫组化检测细胞核增殖抗原(PCNA)表达。结果:OEC-WFDC2-si组皮下移植瘤的生长速度显著慢于OEC组和OEC-mock组,差异有统计学意义(P<0.05)。OECWFDC2-si组首次出现可见肿瘤的时间晚于OEC组和OEC-mock组,差异有统计学意义(P<0.05)。移植后第23天,OEC-WFDC2-si组的肿瘤重量、体积和细胞核增殖抗原(PCNA)表达均低于对照组,差异有统计学意义(P<0.05)。实验组和对照组均未发现淋巴结转移。结论:WFDC2基因沉默显著抑制人卵巢癌SKOV3细胞株在裸鼠体内的生长,WFDC2基因可能在人卵巢浆液性上皮癌进展中起重要作用。Objective:To establish subcutaneous transplanted model of human ovarian carcinoma in nude mice with cell lines with stablely lentiviral WFDC2 gene sequence of small interfering siRNA and to explore its influence on tumor growth and metastasis. Methods:Sub- group was labeled by OEC (ovarian epithelial eancinoma cell lines) ,OEC-mock( transfected by mock lentiviral vector) and OEC-WFDC2-si (transfeeted by with stablely lentiviral WFDC2 gene sequence of small interfering siRNA). Tumor volume were detected by continuous meas- urement to draw the tumor growth curve. Tumor volume and weight were measured at the end point of the study, proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry (IHC). Results: The growth rate was slower and time for first visible tumors was earlier for WFDC silencing group (P〈0.05) , tumor weight, volume and PCNA were lower in WFDC2 silencing group than control group,no metastasis was found in any group. Conclusions:It has been confirmed that WFDC2 gene silencing significantly inhibit the growth of ovarian cancer. WFDC2 gene plays a key role in ovarian cancer development involved in regulating the tumor proliferation.

关 键 词:WFDC2基因 人附睾蛋白4 卵巢癌 

分 类 号:R737.31[医药卫生—肿瘤]

 

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