鸦胆子油乳剂对人胃癌细胞株HGC-27细胞迁移和侵袭的体外实验研究  

Experimental study on effects of Brucea javanica oil emulsion on migration and invasion of human gastric cancer cell line HGC-27 cells in vitro

作　　者：张奇峰[1] 

机构地区：[1]南阳市中心医院,河南南阳473000

出　　处：《中国民康医学》2016年第7期52-54,共3页Medical Journal of Chinese People’s Health

摘　　要：目的:探讨鸦胆子油乳剂对人胃癌细胞株HGC-27细胞迁移和侵袭能力的影响及其机制。方法:采用MTT法检测鸦胆子油乳剂对HGC-27细胞增殖的影响。分别采用Transwell迁移和侵袭实验法检测鸦胆子油乳剂对HGC-27细胞迁移和侵袭的影响及其机制。结果:鸦胆子油乳剂能够明显抑制HGC-27细胞的增殖(P<0.05);鸦胆子油乳剂体外能够显著抑制HGC-27细胞的迁移和侵袭,且呈现剂量依赖关系,25üg·ml^(-1)剂量时迁移和侵袭抑制率分别为53.73%和54.41%。结论:鸦胆子油乳剂对HGC-27细胞增殖有明显抑制作用,并可有效抑制人胃癌细胞株HGC-27的迁移和侵袭能力。Objective: To investigate effects of Brucea javanica oil emulsion on invasion and migration ability of HGC-27 cells and its mechanism. Methods: MTT method was used to study the effects of Brucea javanica oil emulsion on HGC-27 cell proliferation. Transwell migration and invasion assay detection were used to investigate the effect of Brucea javanica oil emulsion of hgc-27 cell migration and its mechanism,respectively. Results: Brucea javanica oil emulsion could significantly inhibit the proliferation of HGC-27 cells( P < 0. 05),and could significantly inhibit the migration and invasion of HGC-27 cells in a dose-dependent manner. The inhibition rates of the dose of 25 ug·ml^(-1) of Brucea javanica oil emulsion on the migration and invasion were 53. 73% and54. 41%,respectively. Conclusions: Brucea javanica oil emulsion can significantly inhibit the proliferation of HGC-27 cell,and can effectively inhibit the migration and invasion ability of human gastric cancer cell line HGC-27.

关 键 词：鸦胆子油乳剂 人胃癌细胞株HGC-27细胞 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]