基于侧向层析原理的赭曲霉毒素A快速检测试纸条的研制  

An Ochratoxin A test strip based on a lateral-flow immunochromatographic assay

作　　者：黄巧莲[1] 金庆日[1] 章先[2] 周一媚 陈彦永 徐露凝[1] 杨永春[1] 程昌勇[1] 田广燕[3] 桂海娈 方维焕[1,2] 宋厚辉[1] 

机构地区：[1]浙江农林大学动物科技学院,浙江临安311300 [2]浙江大学动物科学学院,浙江杭州310058 [3]浙江农林大学外国语学院,浙江临安311300

出　　处：《浙江农林大学学报》2016年第3期531-536,共6页Journal of Zhejiang A&F University

基　　金：国家高技术研究发展计划("863"计划)项目(2012AA101602);国家级大学生创新创业训练计划项目(201410341001);浙江省科学技术公益项目(2015C32018);浙江农林大学人才启动基金项目(2013FR054)

摘　　要：针对霉菌毒素精准检测技术的开发对于保障食品安全至关重要。利用胶体金与赭曲霉毒素A单抗偶联物和赭曲霉毒素A-BSA偶联物(OTA-BSA),开发了一种快速检测赭曲霉毒素A的方法。研究结果表明:1当OTA-BSA配制终质量浓度为4.8 g·L^(-1),质控线用羊抗鼠IgG配制终质量浓度为1.5 g·L^(-1),硝酸纤维素(NC)膜的包被量为1.0μL·cm^(-1)时呈现最清晰、最均匀的条带。2赭曲霉毒素A胶体金快速检测试纸条的灵敏度达到1.0μg·L^(-1),检测时间仅为5 min,非常适合现场快速检测。该检测试纸条具有携带方便、灵敏度高、特异性强等优点。由于本方法检测时间短,灵敏度高,与传统的仪器和酶联免疫检测方法相比,在野外和临床检测中更具有推广应用价值。To develop an accurate and precise detection method to screen Ochratoxin A （OTA） contaminants for food safety concerns, a lateral-flow immunochromatographic assay for OTA detection was developed using colloidal nanoparticles, monoclonal OTA antibodies, and the OTA-BSA （bovine serum albumin） conjugate. The test strip was treated with PBS for negative control and OTA standard samples （ranging from 1 to 10 μg·L^-1） for measurement, and 4 replications. Results over the entire growth period showed that （1） the optimized concentration of OTA-BSA was 4.8g·L^-1and goat-anti mouse （IgG） concentration was 1.5 μg·L^-1. The suitable coating volume of OTA-BSA and IgG in Nitrocellulose （NC） membranes was 1.0 μg·L^-1.cmq with （2） a sensitivity for this test strip of 1.0 μg·L^-1. The entire detection procedure for OTA was completed within 5 min. This test strip provided advantages of easy carrying, high sensitivity, and specificity, thereby serving as a suitable and valuable screening tool for field and clinical detection compared to traditional equipment and to the enzyme-linked immunosorbent assay （ELISA）-based method. [Ch, 5 fig. 1 tab. 12 ref.]

关 键 词：动物医学 赭曲霉毒素A 胶体金试纸条 快速检测 侧向层析 

分 类 号：S859.84[农业科学—临床兽医学]