3株牛乳源乳酸菌安全性初步评价  

Safety Evaluation of Three Lactic Acid Bacteria Derived from Milk Orally Administered to Mice

作　　者：李金敏[1] 张志焱[1] 井维芳[1] 刘永[1] 吴焕荣[1] 韩寅[1] 徐海燕[1] 谷巍[1] 

机构地区：[1]山东宝来利来生物工程股份有限公司,山东泰安271000

出　　处：《乳业科学与技术》2016年第2期4-7,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

摘　　要：通过小鼠的7 d急性毒性实验及30 d喂养实验,对源自牛乳中的3株肠球菌LQ-R-16-1、FC-3-7、FC-3-2进行安全性初步评价。分别对清洁级昆明小白鼠进行7 d及30 d连续灌胃不同剂量的肠球菌LQ-R-16-1、FC-3-7、FC-3-2,实验期间观察小鼠体征变化、计算日增体质量,最后通过大体解剖,观察脏器变化、脏器细菌易位情况并称质量计算脏器指数,脏器细菌易位情况。结果表明:7 d急性毒性实验及30 d喂养实验中各实验组小鼠日增体质量、脏器指数与对照组之间无显著性差异(P>0.05),30 d喂养实验结束后未发现脏器之间有细菌易位现象。初步表明,肠球菌LQ-R-16-1、FC-3-7、FC-3-2安全,无毒副作用。Objective： To assess the safety of 3 enterococci strains （LQ-R-16-1, FC-3-7 and FC-3-2） originating from milk in mice through 7-day acute oral toxicity test and 30-day feeding test. Methods： Kunming mice of clean grade were subjected to intragastric administration of different doses of enterococci and during the test, the mice were observed for changes in physical signs and daily weight gain; afterwards, they were anatomized to observe organ changes and bacterial translocation and calculate organ indices. Results： Acute toxicity and feeding tests exhibited no significant difference in daily weight gain or organ indices compared with the control group （P 〉 0.05）, and after the 30-day feeding test bacterial translocation was found not to occur. Conclusion： Enterococci LQ-R-16-1, FC-3-7, FC-3-2 are safe to consume, having no toxic side effects.

关 键 词：乳酸菌 急性毒性 30 d喂养 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]