高效液相色谱法同时测定液态乳中的2种紫外吸收剂和2种抗氧化剂  

Simultaneous Determination of 2 Ultraviolet Absorbers and 2 Antioxidants in Milk by High Performance Liquid Chromatography

作　　者：张大原 李挥[1] 张兰天[1] 艾连峰[3] 

机构地区：[1]河北省食品检验研究院,河北省食品安全重点实验室,河北石家庄050091 [2]河北衡水中学,河北衡水053000 [3]河北出入境检验检疫局,检验检疫技术中心,河北石家庄050051

出　　处：《乳业科学与技术》2016年第2期17-20,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：河北省科技计划项目(15275505D)

摘　　要：建立液态乳中2种紫外吸收剂（UV-326和UV-1577）和2种抗氧化剂（AO-1010、AO-2246）的高效液相色谱测定方法。前处理采用乙腈提取液态乳中目标化合物，样品用正己烷提取；检测经Agilent HC—C。反相色谱柱，以乙腈一水作为流动相，梯度洗脱程序进行分离，280nm波长下检测。结果表明：分析物在0．05-50μg／mL质量浓度范围内线性关系良好，线性相关系数〉0．9995，方法检出限为0．02～0．5mg／kg。方法在3个水平的加标回收率为69．08％～115．87％，相对标准偏差为0．43％-9．94％。该方法操作简单、灵敏度高、重现性好，适用于液态乳中紫外吸收剂和抗氧化剂的同时测定。A method was developed for the simultaneous determination of 2 ultraviolet absorbers UV-326, UV-1577 and 2 antioxidants AO-1010 and AO-2246 in milk by high performance liquid chromatography. Samples were extracted with acetonitrile. The effective separation of the target analytes was accomplished on Agilent HC-C8 （150 mm - 4.6 mm, 5.0 μm） column with gradient elution procedure using a mixture of water and acetonitrile as the mobile phase. External calibration was used for quantitative determination at detection wavelength of 280 nm. The linearity of 4 analytes were satisfactory with a correlation coefficient of 〉 0.9995 at concentrations ranging from 0.05 to 50 ktg/mL. The limits of detection of the method were 0.02-0.5 mg/kg, and the recoveries at different spiked levels and precision （RSD） were 69.08%-115.87% and 0.43%4.94%, respectively. The method is simple, sensitive, reproducible and suitable for the simultaneous determination of 2 ultraviolet absorbers and 2 antioxidants in milk.

关 键 词：高效液相色谱 紫外吸收剂 抗氧化剂 液态乳 

分 类 号：O657.72[理学—分析化学]