环(组氨酸-脯氨酸)二肽的高温高压辅助合成工艺优化（英文）  

Optimization of High Pressure/Temperature Assisted Synthesis of Cyclo(His-Pro)

作　　者：张艳荣[1] 樊红秀[1] 刘婷婷[1] 刘鸿铖[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品科学》2016年第8期18-25,共8页Food Science

基　　金："十二五"国家科技支撑计划项目(2013BAD16B08);国家高技术研究发展计划(863计划)项目(2011AA100805)

摘　　要：将高温高压技术应用于环(组氨酸-脯氨酸)二肽(cyclo(His-Pro),CHP)的制备。以二肽甲酯盐酸盐为原料,采用高温高压辅助环化法在水中制备CHP,通过单因素试验考察了各种反应变量如压力、时间、溶液p H值以及底物质量浓度对CHP产率的影响,采用正交试验对高温高压辅助环化工艺条件进行优化。采用超高效液相色谱法和电喷雾质谱技术对产物中的CHP进行定量和定性分析。结果表明,最佳的反应条件为:反应压力0.20 MPa、反应时间3.5 h、溶液p H 6.0、底物质量浓度15 mg/m L。在此条件下可以得到较高产率(91.35%)的CHP,并且反应中没有观察到消旋。与传统的甲醇回流法相比,本研究的环化方法省时、高效、环保,并且能够得到较高的产率,同时产物没有发生消旋。In this study the synthesis of cyclo（His-Pro）was investigated under high pressure/temperature condition.Using dipeptide methyl ester hydrochloride as starting material,cyclo（His-Pro）was synthesized in water by high pressure/temperature assisted cyclization.Influences of different reaction variables including pressure,time,solution p H and substrate concentration on the yield of cyclo（His-Pro）were investigated by single-factor experiments,and then an orthogonal array design was used to optimize the cyclization conditions.Quantification and identification of cyclo（His-Pro）in the product were carried out by ultra performance liquid chromatography（UPLC）and electro spray ionization mass spectrometry（ESI-MS）.The optimum cyclization conditions were determined as follows：reaction pressure,0.20 MPa;reaction time,3.5 h;solution p H,6.0;and substrate concentration,15 mg/m L.Under such conditions,cyclo（His-Pro）was obtained in excellent yield（91.35%）and no racemization was observed in the reaction process.In contrast to the classic methanol-reflux cyclization,this method is rapid,environmentally friendly and highly efficient.

关 键 词：环(组氨酸-脯氨酸)二肽 高温高压技术 环化反应 环二肽制备 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]