星形胶质细胞连接蛋白43及其半通道在脑缺血再灌注损伤中的作用  

Effects of Connexin 43 and its hemichannels of astrocytes on ischemia reperfusion injury

作　　者：李书玮[1] 马爱华[1] 王学禹[1] 孙文秀[1] 

机构地区：[1]山东大学附属省立医院小儿神经科,济南250021

出　　处：《临床神经病学杂志》2016年第2期128-132,共5页Journal of Clinical Neurology

基　　金：英国皇家学会(2010/R218);山东省医药卫生科技计划(2011HZ072)

摘　　要：目的探讨星形胶质细胞连接蛋白43(Cx43)及其半通道在缺血再灌注(IR)损伤中的作用。方法 32只Wistar鼠被随机分为IR 0 h组、IR 4 h组和IR 24 h组和对照组,每组8只鼠。用免疫组化和Western印迹法检测各组大鼠星形胶质细胞Cx43、半通道抗体1(HC1)和半胱天冬酶3(Casp3)的表达。在氧葡萄糖剥夺-再恢复(OGDR)0 h,4 h和24 h,用MTT法检测星形胶质细胞活性,用免疫组化和Western印迹法检测Cx43,HC1和Casp3表达的改变。结果大鼠星形胶质细胞中HC1的表达明显少于Cx43的表达。与对照组比较,IR 4 h组大鼠脑组织中Cx43、HC1和Casp3的表达明显增加(均P<0.05),而IR 0 h组和IR 24 h组却没有明显的变化。OGDR后星形胶质细胞cell line和Psup细胞中Cx43、HC1和Casp3的表达在OGDR 4h组明显高于对照组(均P<0.05),在OGDR 24 h组则与对照组差异无统计学意义。OGDR后shRNA星形胶质细胞中Cx43、HC1和Casp3的表达无统计学意义上变化。结论 Cx43及HCl在星形胶质细胞凋亡过程中起到了促进作用,这可能是IR损伤发生及发展的机制之一。Objective To observe the effects of Connexin 43（ Cx43） and its hemichannels of astrocytes on ischemia reperfusion（ IR） injury. Methods Thirty-two wistar rats randomly divided into IR 0 h group,IR 4 h group,IR 24 h group and control group,with 8 rats in each group）. The Cx43,hemichannel（ HC1） and Caspase 3（ Casp3） in rat astrocytes were examined by immunohistochemistry and western-blotting. The viability of astrocytes was assessed by MTT assay,and the expression of Cx43,HC1 and Casp3 was detected with immunohistochemical and Western blotting methods at 0 h,4 h,24 h after oxygen-glucose deprivation reoxygenation（ OGDR）. Results The expression of HC1 in rat astrocytes was significantly less than CX43. Compared with the control group,the expression of Cx43,HC1 and Casp3 in rats＇ brain were significantly increased in IR 4 h group（ all P〈0. 05）,but there were no significant changes in IR 0 h group and IR 24 h group. The expression of Cx43,HC1,Casp3 in astrocyte cell line and Psup cells followed by OGDR insult was significantly increased in OGDR 4 h group than the control group（ all P〈0. 05）,but there were no statistical differences in OGDR 24 h group. The expression of Cx43,HC1 and Casp3 had no statistical differences in the shRNA astrocytes after the treatment of OGDR. Conclusions Cx43 and HC1 may play a promoting role in the astrocyte apoptosis process. It may be one of the mechanisms of the occurrence and development of IR.

关 键 词：缝隙连接 半通道 星形胶质细胞 缺血再灌注损伤 氧葡萄糖剥夺-再恢复 

分 类 号：R743[医药卫生—神经病学与精神病学]