Anti-inflammatory effect of Heliotropium indicum Linn on lipopolysaccharide-induced uveitis in New Zealand white rabbits  

作　　者：Samuel Kyei George Asumeng Koffuor Paul Ramkissoon Elvis Ofori Ameyaw Emmanuel Akomanin Asiamah 

机构地区：[1]Discipline of Optometry, School of Health Sciences, College of Health Sciences, University of Kwa Zulu-Natal [2]Department of Optometry, School of Allied Health Sciences,University of Cape Coast, PMB [3]Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences,Kwame Nkrumah University of Science and Technology,PMB [4]Department of Biomedical and Forensic Sciences, School of Allied Health Science, University of Cape Coast, PMB [5]Department of Pathology, Komfo Anokye Teaching Hospital

出　　处：《International Journal of Ophthalmology(English edition)》2016年第4期528-535,共8页国际眼科杂志（英文版）

基　　金：Partly supported by University of Cape Coast

摘　　要：AIM： To investigate the anti-inflammatory effect of an aqueous whole plant extract of Heliotropium indicum（HIE） on endotoxin-induced uveitis in New Zealand white rabbits.·METHODS： Clinical signs of uveitis including flares,iris hyperemia and miosis, were sought for and scored in1.0 mg/kg lipopolysaccharide（LPS）-induced uveitic rabbits treated orally with HIE（30-300 mg/kg）,prednisolone（30 mg/kg）, or normal saline（10 m L/kg）. The number of polymorphonuclear neutrophils infiltrating, the protein concentration, as well as levels of tumor necrosis factor-α（TNF-α）, prostaglandin E2（PGE2）, and monocyte chemmoattrant protein-1（MCP-1） in the aqueous humor after the various treatments were also determined. A histopathological study of the anterior uveal was performed.· RESULTS： The extract and prednisolone-treatment significantly reduced（P ≤0.001） both the clinical scores of inflammation（1.0-1.8 compared to 4.40 ±0.40 in the normal saline-treated rabbits） and inflammatory cells infiltration. The level of protein, and the concentrationsof TNF-α, PGE2 and MCP-1 in the aqueous humor were also significantly reduced（P ≤0.001）. Histopathological studies showed normal uveal morphology in the HIE and prednisolone-treated rabbits while normal saline-treated rabbits showed marked infiltration of inflammatory cells.· CONCLUSION： The HIE exhibits anti-inflammatory effect on LPS-induced uveitis possibly by reducing the production of pro-inflammatory mediators.AIM： To investigate the anti-inflammatory effect of an aqueous whole plant extract of Heliotropium indicum（HIE） on endotoxin-induced uveitis in New Zealand white rabbits.·METHODS： Clinical signs of uveitis including flares,iris hyperemia and miosis, were sought for and scored in1.0 mg/kg lipopolysaccharide（LPS）-induced uveitic rabbits treated orally with HIE（30-300 mg/kg）,prednisolone（30 mg/kg）, or normal saline（10 m L/kg）. The number of polymorphonuclear neutrophils infiltrating, the protein concentration, as well as levels of tumor necrosis factor-α（TNF-α）, prostaglandin E2（PGE2）, and monocyte chemmoattrant protein-1（MCP-1） in the aqueous humor after the various treatments were also determined. A histopathological study of the anterior uveal was performed.· RESULTS： The extract and prednisolone-treatment significantly reduced（P ≤0.001） both the clinical scores of inflammation（1.0-1.8 compared to 4.40 ±0.40 in the normal saline-treated rabbits） and inflammatory cells infiltration. The level of protein, and the concentrationsof TNF-α, PGE2 and MCP-1 in the aqueous humor were also significantly reduced（P ≤0.001）. Histopathological studies showed normal uveal morphology in the HIE and prednisolone-treated rabbits while normal saline-treated rabbits showed marked infiltration of inflammatory cells.· CONCLUSION： The HIE exhibits anti-inflammatory effect on LPS-induced uveitis possibly by reducing the production of pro-inflammatory mediators.

关 键 词：monocytechemotaticprotein-1 prostaglandin E2 tumor necrosis factor-α polymorphonuclear neutrophil iris hyperemia prednisolone heliotropium uveitis 

分 类 号：R773.9[医药卫生—眼科]