散发性乳腺癌患者乳腺癌易感基因1mRNA与蛋白的表达变化差异及意义  

Differential expression of breast cancer susceptibility gene 1 mRNA and protein in sporadic breast cancer

作　　者：张楠[1] 孙刚[1] 马斌林[1] 

机构地区：[1]新疆医科大学附属肿瘤医院乳腺头颈外科,乌鲁木齐830011

出　　处：《中华实验外科杂志》2016年第4期884-887,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81360391）

摘　　要：目的探讨散发性乳腺癌患者乳腺癌易感基因1（BRCA1）mRNA与蛋白的表达变化及差异。方法应用荧光定量聚合酶链反应（FQ—PCR）检测正常乳腺组织（n=40）与散发性乳腺癌组织（n=80）中BRCA1mRNA的表达量变化；用免疫组织化学方法检测BRCA1蛋白表达变化及定性观察BRCA1蛋白亚细胞定位的变化，分析BRCA1与散发性乳腺癌发生发展的相关性。结果（1）散发性乳腺癌组织中BRCA1mRNA表达水平（0．759±0．440）低于正常乳腺组织（1．530±0．810），差异有统计学意义（P〈0．05），原位癌伴微小浸润组（0．956±0．340）与浸润癌组BRCA1（0．561±0．443）表达差异有统计学意义（P〈0．01），原位癌组表达水平高于浸润性乳腺癌组。（2）BRCA1蛋白在正常乳腺组织高表达，在乳腺癌组织中表达降低，其阳性率分别为95％和46％，差异有统计学意义（，=27．040，P〈0．01）；浸润性乳腺癌组织中BRCA1蛋白表达水平低于导管原位癌伴微小浸润组织，差异有统计学意义（x^2=4．037，P〈0．05）。本研究还观察到BRCA1蛋白在正常乳腺组织中表达于细胞核，在乳腺癌组织中可以见到BRCA1蛋白在细胞核与细胞质中均表达，且在细胞核上的表达率降低。结论BRCA1mRNA与蛋白在散发性乳腺癌疾病进展过程中表达变化存在差异。Objective To investigate the changes in mRNA and protein expression of breast canc- er susceptibility gene 1 （ BRCA1 ） during the development and progression of sporadic breast cancer. Meth- ods BRCA1 mRNA expression in sporadic breast cancer tissues （ n = 80） and normal breast tissues （ n = 40） was detected by real - time fluorescent quantitative polymerase chain reaction （ FQ - PCR）. The BRCAI protein expression and subcellular location were determined by immunohistochemistry. The correla- tion between BRCA1 and development and progression of sporadic breast cancer was analyzed. Results （ 1 ） BRCA1 mRNA expression was lower in breast cancer tissues （0. 759 ± 0. 440 ） than in normal breast tissues （1. 530 ± 0. 810）, and significantly higher in carcinoma in situ （with minimal invasion） （0. 956 ± 0. 340） than in invasive breast cancers （0. 561 ± 0. 443, P 〈 0.01 ）. （2） BRCA1 protein was highly ex- pressed in normal breast tissues, and lowed expressed in breast cancer tissues （positive rate： 95% vs. 46% ,x^2 = 27. 040 ,P 〈 0. 01 ）. BRCA1 expression was also significantly lower in invasive breast cancer tis- sues than in ductal carcinoma in situ （with minimal invasion） （x^2 =4. 037 ,P 〈0. 05）. We also observed alterations in subcellular location of BRCA1 protein confined in the nucleus in normal breast tissues, and diffused in both nucleus （ at decreased level） and the cytoplasm in breast cancer tissues. Conclusion BRCA1 mRNA and protein expression altered with the development and progression of sporadic breast cancer.

关 键 词：乳腺癌易感基因1 散发性乳腺癌 荧光定量聚合酶链反应 

分 类 号：R737.31[医药卫生—肿瘤]