A mouse model of weight-drop closed head injury:emphasis on cognitive and neurological deficiency  

作　　者：Igor Khalin Nor Laili Azua Jamari Nadiawati Bt Abdul Razak Zubaidah Bt Hasain Mohd Asri bin Mohd Nor Mohd Hakimi bin Ahmad Zainudin Ainsah Bt Omar Renad Alyautdin 

机构地区：[1]Faculty of Medicine and Defence Health,National Defence University of Malaysia,Kem Sungai Besi,Kuala Lumpur,Malaysia [2]Chemistry Department,Centre for Defence Foundation Studies,National Defence University of Malaysia,Kem Sungai Besi,Kuala Lumpur,Malaysia [3]Department of Civil Engineering,Faculty of Engineering,National Defence University of Malaysia,Kem Sungai Besi,Kuala Lumpur,Malaysia [4]Centre for Research and Innovation Management,National Defence University of Malaysia,Kem Sungai Besi,Kuala Lumpur,Malaysia [5]Scientific Centre for Expertise of Medical Application Products,Petrovsky Blvd,Moscow,Russia

出　　处：《Neural Regeneration Research》2016年第4期630-635,共6页中国神经再生研究（英文版）

基　　金：supported by a grant from the Ministry of Higher Education of Malaysia,No.RAGS/2013/UPNM/SKK/01/2

摘　　要：Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of potential therapeutics,which are effective in the treatment of TBI,animal models of TBI should be established and standardized.In this study,we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model.We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice.Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury.Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of potential therapeutics,which are effective in the treatment of TBI,animal models of TBI should be established and standardized.In this study,we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model.We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice.Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury.Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.

关 键 词：nerve regeneration traumatic brain injury neurological severity score passive avoidance weightdrop injury model C57BL/6 mice neural regeneration 

分 类 号：R749.12[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]