两种外源性酶对牙本质粘接界面的老化作用初探  

Effects of exogenous enzymes on the degradation of adhesive-dentin interfaces

作　　者：邓东来[1] 杨宏业[2] 郭景梅[2] 黄翠[3] 甘靖[2] 宋芳芳[2] 

机构地区：[1]武汉大学口腔医学院中心门诊,430022 [2]武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,430022 [3]武汉大学口腔医学院修复科,430022

出　　处：《中华口腔医学杂志》2016年第4期230-234,共5页Chinese Journal of Stomatology

基　　金：教育部科学技术研究重点（重大）项目（2013000964658）

摘　　要：目的 比较两种外源性酶对牙本质粘接界面的老化效果,探讨酶解作为牙本质粘接耐久性实验方法的可行性.方法 选取完整无龋离体第三磨牙40颗,用随机数字表法分为两组（每组20颗）,去除冠部釉质,暴露中部牙本质,分别用全酸蚀粘接剂（A组）和自酸蚀粘接剂（B组）粘接,树脂塑形,切割为横截面积为0.9 mm×0.9 mm的牙本质-树脂粘接试件.每组再分为4个亚组（每亚组20个试件）：即刻亚组（水储存24 h后即刻实验）、水储存老化亚组（水储存6个月）、胶原酶老化亚组（溶组织梭菌胶原酶老化12周）、酯酶老化亚组（胆固醇酯酶老化12周）.各亚组相应处理后,分别测试微拉伸强度（即粘接强度,每亚组15个试件）;采用体式显微镜和背散射扫描电镜观察试件断面断裂模式,并观察粘接界面纳米渗漏情况（每亚组5个试件）.结果 全酸蚀粘接剂组和自酸蚀粘接剂组3个老化亚组的粘接强度均显著低于即刻亚组（P＜0.05）;使用全酸蚀粘接剂的胶原酶老化亚组粘接强度[（19.6±3.5） MPa]显著低于水储存老化亚组[（23.4±4.2）MPa]和酯酶老化亚组[（24.2±4.2）MPa]（P＜0.05）;使用自酸蚀粘接剂的3个老化亚组粘接强度差异无统计学意义（P＞0.05）.扫描电镜显示,相对于即刻亚组,使用两种粘接剂的3个老化亚组粘接界面银颗粒沉积明显增多.体式显微镜显示,两种粘接剂各亚组均以粘接界面断裂为主要断裂模式.结论 溶组织梭菌胶原酶和胆固醇酯酶均能降低牙本质粘接界面的粘接强度,达到老化的目的;模拟口腔生物环境的外源性酶老化可作为新的粘接界面老化方式.Objective To compare the effects of exogenous enzymes on the degradation of adhesive-dentin interface.Methods Forty molars were sectioned to expose the middle-coronal dentin surface and randomly divided into two adhesive systems：an etch-and-rinse adhesive Adper Single Bond 2 and a self-etching adhesive G-Bond.After composite building up,the specimens were then randomly assigned to four groups（n=5 for each group） as follows：group 1,24 h of water storage（the control group）;group 2,six months of water storage;group 3,twelve weeks storage in artificial saliva containing clostridium histolyticum collagenase;group 4,twelve weeks storage in artificial saliva containing cholesterolesterase.The microtensile bond strengths（MTBS） were then tested.The failure modes and nanoleakage were analyzed.Results After aging treatments,the three aging groups showed significantly lower MTBS compared with the control group in both adhesive systems（P〈0.05）.For etch-and-rinse adhesive Adper Single Bond 2,the MTBS of group 3（[19.6±3.5] MPa） was lower than that of group 2（[23.4±4.2] MPa） and group 4（[24.2±4.2] MPa） （P〈0.05）.For self-etching adhesive G-Bond,there was no difference on MTBS among different aging groups （P〉0.05）.SEM observation showed that,compared with the control group,water storage（group 2） and the exogenous enzymes（group 3 and 4） increased the nanoleakage expression（silver deposition） of both adhesive systems.Adhesive failure was the predominant fracture modes in all groups.Conclusions Storage in artificial saliva containing clostridium histolyticum collagenase or cholesterol esterase could be used to accelerate the degradation process of adhesive-dentine interface.

关 键 词：胶原酶类 固醇酯酶 牙粘合 粘接耐久性 

分 类 号：R783.1[医药卫生—口腔医学]