黄瓜单性结实候选基因预测与表达分析  

Prediction and Expression Analysis of Parthenocarpy Candidate Genes in Cucumber

作　　者：张婷[1] 武喆[1] 张开京[1] 徐建[1] 娄群峰[1] 李季[1] 陈劲枫[1] 

机构地区：[1]南京农业大学园艺学院/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《核农学报》2016年第2期224-230,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家重点基础研究发展计划"973"项目(2012CB113904);农业部行业专项(201403032);国家自然基金青年基金(31301781)

摘　　要：为发掘黄瓜单性结实基因并通过表达分析预测相关基因的功能,以黄瓜单性结实性状QTL定位结果为基础,对主效QTL区段内的基因进行生物信息学分析,发现118个基因与细胞周期、细胞生长以及激素信号传导等生物学过程有关。结合黄瓜单性结实转录组研究结果,共筛选出10个在黄瓜单性结实过程中转录水平有剧烈变化的基因。利用q RT-PCR技术分析这些基因在授粉、未授粉及单性结实的黄瓜果实发育过程中的转录变化,结果表明,Cs CRT1、Cs LON、Cs TRAPPC4、Cs RABEPK和Cs POT基因在单性结实坐果过程中特异性的上调表达,Cs5NG4基因在单性结实和授粉结实过程中呈相反表达趋势,因此推测这6个基因为黄瓜单性结实候选基因。本研究为进一步利用候选基因资源改良现有黄瓜栽培品种的单性结实性奠定了基础。The purpose of this paper is to explore parthenocarpic genes of cucumber and preliminarily identify the function of the genes by transcriptional study. In the study,based on localization of the major parthenocarpy QTLS,118 genes were identified referred to biological process e. g. cell cycle,cell growth,hormone signal transduction. Coanalyzing with the transcriptome data,10 different expression genes were selected and their potential function were annotated by KEGG. The expression patterns of the 10 genes responding to differently developed cucumber fruits were analyzed by real-time RT-PCR. Only the Cs CRT1,Cs LON,Cs TRAPPC4,Cs RABEPK and Cs POT have up-regulate expressed during parthenocarpy fruit set; the Cs5NG4 expressed contrary between the parthenocarpy and pollination progress. These may attribute to improve the available cultivars of facility cucumber through utilizing of candidate gene resources.

关 键 词：黄瓜 单性结实 基因预测 表达分析 

分 类 号：S642.2[农业科学—蔬菜学]