RNA干扰S100A4基因表达对肺癌SPC-A-1细胞侵袭能力的影响  

Effect on Invasion and Proliferation of Human Lung Cancer Cell SPC-A-1 by RNA Inhibiting S100A4 Gene Expression

作　　者：王建[1] 邵中夫[1] 徐刚[1] 李晓宁[1] 周天骏[1] 

机构地区：[1]广州医科大学附属肿瘤医院胸外科,广东广州510095

出　　处：《现代医用影像学》2016年第1期7-12,共6页Modern Medical Imageology

基　　金：广东省医学科研基金立项资助项目(2013-36)

摘　　要：目的:研究小干扰RNA抑制高迁移族蛋白s10基因表达对肺癌细胞SPC-A-1增值和侵袭能力的影响。材料与方法:设计合成靶向S100A4基因的siRNA,采用阳离子脂质体试剂瞬时转染肺癌细胞株SPC-A-1,利用实时荧光定量PCR和Western blot检测RNA干扰后S100A4基因的沉默效果,评价siRNA设计的合理性及RNA干扰抑制S100A4表达的有效性;用细胞活力计数仪测定3组细胞活力;分别在RNA干扰后24、48、72和96 h应用MTT实验检测细胞生存状态,计算生长抑制率并绘制生长曲线;应用boyden chamber法检测侵袭能力的差异。结果:靶向S100A4的siRNA成功抑制肺癌细胞株SPC-A-1中S100A4基因及蛋白表达,细胞活力检测仪测定RNA干扰48 h后,细胞活力显著下降;MTT测定肺癌细胞生长受到明显抑制;boyden chamber小室细胞侵袭实验提示肺癌穿膜细胞数明显减少。结论:应用siRNA技术能有效地抑制S100A4基因的表达,同时有效抑制SPC-A-1细胞的体外增值和侵袭,为肺癌的生物学治疗提供了新思路。Purpose： To study the effects and mechanism of S100A4 small interfering RNA（ siRNA） on invasion and proliferation of human lung cancer cell. Materials and Methods： After lung cancer cell line SPC-A-1 were transfected by S100A4 siRNA,the mRNA and protein of PRL21 were determined by real time PCR and western blot assay,respectively. The anchorage-independent growth was exmined by clonformation in soft agar,and invasion ability was evaluated by boyden chamber model. Results： The siRNA could down-regulate the level of mRNA and protein of S100A4. Suppression of of S100A4 expression could inhibit invasion and proliferation ability in human lung cancer cell SPC-A-1. Conclusion： S100A4 gene might play an important role in development of human lung cancer,and down-regulation by S100A4 siRNA could inhibit invasion and proliferation of human lung cancer cell.

关 键 词：肺癌 S100A4 增殖 侵袭 RNA干扰 

分 类 号：R734.2[医药卫生—肿瘤]