GHR基因遗传变异及其对中国西门塔尔牛胴体性状的效应分析  

Genetic distribution of GHR gene and its association with carcass traits in Chinese Simmental cattle

作　　者：武秀香[1] 杨章平[2] 毛永江[2] 施雪奎[3] 黄必志[4] 王安奎[4] 亏开兴[4] 

机构地区：[1]徐州医学院生物学教研室,江苏徐州221004 [2]扬州大学动物科学与技术学院,江苏扬州225009 [3]苏州大学科技处,江苏苏州215006 [4]云南省草地动物科学研究院,云南昆明650212

出　　处：《扬州大学学报（农业与生命科学版）》2015年第4期31-34,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家高技术研究发展计划项目(863-2013AA102505-5)

摘　　要：为探索生长激素受体(GHR)基因在中国黄牛不同群体中的变异以及GHR基因作为影响牛肉质性状候选基因的可能性,采用PCR-SSCP技术对雷琼牛、云南高峰牛、BMY牛及中国西门塔尔牛共367头个体GHR基因部分外显子遗传变异进行检测,分析群体遗传特征,并运用SPSS17.0软件中GLM模型分析所检测的遗传变异与中国西门塔尔牛部分肉质性状的关联性。结果表明:在GHR基因DNA序列中发现T229C多态位点,雷琼牛群体处于Hardy-Weinberg平衡状态,其他群体偏离平衡。对GHR基因T229C位点与胴体性状关系分析表明:该位点对肌肉生长性状效应显著,对宰前活重、胴体重、屠宰率、胴体产肉率、净肉率、肉骨比、头与蹄重、脂肪覆盖率也有显著影响。结果提示GHR基因可作为牛屠宰及肉质性状的候选基因。To explore the distribution of growth hormone receptor gene（GHR）in Chinese native cattle populations and beef cattle populations,and to evaluate it as a candidate molecular marker-associated with beef qualities,PCR-SSCP method was used to detect the polymorphism of GHRin 367 heads from four cattle populations,including Leiqiong,Yunnan highpump,BMY and Chinese Simmental cattle populations.The association between SNP locus and some meat straits was analyzed by general linear model（GLM）in SPSS program.The results showed that three genotypes,CC/CT/TT,were detected at site T229 C,and only Leiqiong breed achieved Hardy-Weinberg equilibrium.The cattle with TT genotype had higher performance in slaughter weight,dressing percentage,carcass weight,rate of carcass meat,net meat rate,rate of fat cover straits,ratio of meat by bones,head and hoof weight（P〈0.05）.Site T229 Cof GHRgene could be used as genetic marks for beef carcass trait.

关 键 词：中国黄牛 GHR基因 遗传变异 胴体性状 

分 类 号：S823.2[农业科学—畜牧学]