B7-H4促进B细胞凋亡  

Effect of B7-H4 on Apoptosis of B Cells in Tonsils

作　　者：时正朋 陈翰卿[1] 孙静[2] 樊明月[3] 傅丰庆[4] 

机构地区：[1]苏州大学医学部基础医学与生物科学学院,江苏苏州215006 [2]苏州卫生职业技术学院,江苏苏州215009 [3]苏州大学附属儿童医院,江苏苏州215003 [4]苏州大学附属第一医院江苏省临床免疫研究所,江苏苏州215007

出　　处：《中国血液流变学杂志》2015年第3期251-254,共4页Chinese Journal of Hemorheology

基　　金：国家自然科学基金资助项目（31170834）;江苏青蓝工程学术带头人培养对象（孙静）

摘　　要：目的：检测B7-H4受体在B细胞表面的表达，研究B7-H4蛋白对细胞增殖和细胞凋亡的影响，探讨B7-H4通过未知受体传递的下游信号机制。方法分选扁桃体中CD19＋B细胞，流式细胞术检测CD19＋B细胞的受体的表达情况；采用CCK8法和annexinⅤ/PI凋亡试剂盒分别检测细胞增殖和细胞凋亡；进一步采用Western blot检测凋亡信号通路的AKT、MAPK信号通路的磷酸化变化。结果 CD19＋B细胞表面存在B7-H4未知受体，经LPS与anti-CD40刺激受体表达增加；B7-H4蛋白抑制B细胞增殖，促进B细胞的凋亡，并抑制AKT和MAPK的信号通路。结论 B7-H4蛋白通过B细胞表面表达的受体促进细胞凋亡，这为B7-H4未知受体的寻找及其进一步介导的信号研究奠定了理论基础。Objective To analyze the expression of B7-H4 unknown receptor on B cells, and investigate the effect of B7-H4 protein on cell proliferation and apoptosis as well as the mechanism of B7-H4 signal through the unknown receptor.Methods The CD19＋B cells were separated and sorted from tonsil usingfi coll and magnetic beads. The unknown receptor was detected byfl ow cytometry. Cell proliferation and apoptosis were analyzed by CCK8 and annexinⅤ/PI staining kit. The phosphorylation level of AKT and MAPK were detected by Western blot.Results The expression of B7-H4 was detected on the surface of B cells and induced by LPS and anti-CD40 mAb stimulation. B7-H4 could inhibit B cell proliferation and improve cell apoptosis rate, may through the AKT and MAPK signal pathway.Conclusion B7-H4 could induce B cell apoptosis through the unknown receptor. This study could be the basis for the research of B7-H4 unknown receptor and the mechanism of the B7-H4 function.

关 键 词：扁桃体 B细胞 B7-H4 B7-H4受体 凋亡 磷酸化 

分 类 号：R730.3[医药卫生—肿瘤]