结核分枝杆菌mpt64基因突变研究  

Study on mpt64 gene mutation of Mycobacterium tuberculosis

作　　者：毛欣茹 张诗蒙[2] 尹小毛[3] 

机构地区：[1]南方医科大学南方医院,广东广州510515 [2]深圳市蛇口人民医院,广东深圳518067 [3]南方医科大学第五附属医院,广东广州510900

出　　处：《实验与检验医学》2016年第2期137-139,共3页Experimental and Laboratory Medicine

基　　金：广东省省级科技计划项目(2014A020212173);南方医科大学科研启动计划项目(C1031780)

摘　　要：目的分析结核分枝杆菌(Mycobacterium tuberculosis,MTB)mpt64基因突变情况。方法提取MTB标准株H37Rv和45株MTB临床株的DNA,扩增mpt64基因并进行序列分析。结果 MTB标准株H37Rv和44株MTB临床株的mpt64基因无突变,1株MTB临床分离株的mpt64基因存在63个碱基的缺失突变。结论结核分枝杆菌mpt64基因突变频率低。Objective To investigate the mpt64 gene mutation situation of Mycobacterium tuberculosis（MTB）. Methods DNA extraction was performed from reference strain H37Rv and 45 clinical MTB isolation strains. Then the amplification and sequencing of mpt64 gene were accomplished. Results No mution of mpt64 gene was found in H37Rv and clinical isolation strains except for one clinical MTB strain had a deletion of 63 nucleotides. Conclusion The mutation prevalence of mpt64 gene of MTB is low.

关 键 词：结核分枝杆菌 mpt64基因 突变分析 非结核分枝杆菌 

分 类 号：R378.91[医药卫生—病原生物学] Q523[医药卫生—基础医学]