小白蒿总黄酮对大鼠腹腔白细胞内白三烯B4、5-羟二十碳四烯酸、游离钙和环一磷酸腺苷水平的影响  

Effect of Artemisia frigida Total Flavonoids on the Levels of LTB4,5-HETE,Ca^(2+) and Cyclic AMP in Rat Peritoneal Leukocytes

作　　者：花拉[1] 王青虎[1] 代那音台[1] 那仁朝克图[1] 吴荣君 吴杰斯 

机构地区：[1]内蒙古民族大学蒙医药学院,通辽028000

出　　处：《天然产物研究与开发》2016年第4期591-595,600,共6页Natural Product Research and Development

基　　金：国家自然科学基金(8146054)

摘　　要：本文以大鼠胸腔白细胞为材料,采用高效液相色谱法(HPLC)、放免分析法(RIA)和荧光分光光度法分别测定大鼠腹腔白细胞内白三烯B4(LTB4)、5-羟二十碳四烯酸(5-HETE)、环一磷酸腺苷(c AMP)及游离钙水平。结果表明,在2.0~10.0μg/m L浓度范围内,小白蒿总黄酮可剂量依赖性地抑制完整中性白细胞中LTB4的生物合成。另外,小白蒿总黄酮对人工三肽(f MLP)激发的白细胞内游离钙升高具有抑制作用,并促进细胞内c AMP水平提高。小白蒿总黄酮显著影响白细胞的多种功能,这可能与其抗炎作用机理有关。In this study,rat neutrophils from pleural fluid was used as experimental material to determine the levels of LTB4,5-HETE,Ca^2＋and c AMP with HPLC,RIA and Fura-2 / AM,respectively. As a results,Artemisia frigida total flavonoids inhibited the formation of LTB4 from the intact neutrophils with its concentration increasing from 2. 0 to 10. 0mg / L,and inhibited the Ca^2＋ stimulated by f MLP and promoted the improvement of c AMP in the cells. The effects of A.frigida total flavonoids on leukocyte function may be the mechanisms of its anti-inflammatory action.

关 键 词：小白蒿 总黄酮 白三烯B4 游离钙 环一磷酸腺苷 

分 类 号：R914[医药卫生—药物化学]