利用TCGA数据集分析AKT3在胃癌中的表达及临床意义  

Analysis of Clinical Significance of AKT3 Expression in Gastric Cancer Utilizing TCGA Datasets

作　　者：王硕[1] 李智[1] 郑春雷[1] 曲秀娟[1] 刘静[1] 曲晶磊[1] 车晓芳[1] 刘云鹏[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤内科,沈阳110001

出　　处：《中国医科大学学报》2016年第5期398-401,共4页Journal of China Medical University

基　　金：国家自然科学基金(81302023);国家科技重大新药创制(2013ZX09303002);辽宁省科学技术计划(2014225013)

摘　　要：目的研究AKT3在胃癌中的表达情况,预测并探讨AKT3参与肿瘤发生发展的可能机制及临床价值。方法从癌症基因组图谱(TCGA)数据库收集胃癌数据集,下载AKT3基因表达谱资料及临床信息资料。分析AKT3表达与胃癌临床病理学参数的相关性及对预后的影响。采用基因集富集分析(GSEA)方法预测AKT3的调控基因。结果 AKT3表达水平与肿瘤浸润深度(P=0.001)、TNM分期(P=0.049)、分化程度(P<0.001)相关,肿瘤恶性程度越高,AKT3表达越高。AKT3高表达患者总生存期明显短于低表达患者(P<0.001)。AKT3高表达样本富集了细胞黏附、骨架蛋白调控、紧密连接、TGF-β通路等基因集。结论在胃癌中,AKT3高表达是一种预后不良因素,可以作为预测患者转移发生、判断预后的有效生物标志物。Objective To investigate the prognostic value of AKT3 expression in gastric cancer. Methods AKT3 expression data in The Cancer Genome Atlas （TCGA） datasets and its clinical information were downloaded. Statistically assessed was performed for relationship with clinicopatho- logical factors and prognosis. Gene set enrichment analysis （GSEA） was used to predict the gene sets modulated by AKT3. Results The expres- sion of AKT3 was associated with T stage（P = 0.001 ）, TNM stage（P = 0.049） and differentiation（P 〈 0.001 ）.High level of AKT3 expression indi- cates poor prognosis （P = 0.001 ）. AKT3 could regulate gene sets involving cell adhesion molecule, cytoskeleton regulation, focal adhesion and TGF-β signaling pathway. Condusion AKT3 could be used as a potential prognostic marker and a therapeutic target in gastric cancer.

关 键 词：胃癌 癌症基因组图谱 AKT3 预后 

分 类 号：R735.2[医药卫生—肿瘤]