SRSF1和Survivin在前列腺癌组织中的表达及其与病理分级的相关性  

Correlations between the Expressions of SRSF1 and Survivn and the Pathological Features of Prostate Cancer

作　　者：肖邦明[1] 祝兴旺[1] 董理鸣[1] 刘屹立[1] 王平[1] 

机构地区：[1]中国医科大学附属第四医院泌尿外科,沈阳110032

出　　处：《中国医科大学学报》2016年第5期452-455,共4页Journal of China Medical University

摘　　要：目的检测人丝氨酸/精氨酸富有剪接因子1(SRSF1)和凋亡抑制因子(Survivin)在前列腺癌组织中的表达,并探讨两者的相关性及其与前列腺癌病理分级的关系,从而初步提出前列腺癌治疗中的新靶点。方法采用免疫组织化学SP法检测SRSF1、Survivin蛋白在12例前列腺癌组织和8例良性前列腺增生组织中的表达,分析其与病理分级的关系,并探讨两者的相关性。结果 SRSF1蛋白在前列腺癌组织中的细胞阳性表达率为(76.37±5.06)%,显著高于良性前列腺增生[(11.30±1.09)%,P<0.05],Survivin蛋白在前列腺癌组织中的细胞阳性表达率为(86.93±3.21)%,显著高于良性前列腺增生[(17.67±1.99)%,P<0.05]。SRSF1和Survivin蛋白在前列腺癌组织中的表达与病理Gleason分级呈正相关,并且二者有明显的相关性(P<0.05)。结论SRSF1和Survivin蛋白在前列腺癌组织中均有高表达,比在良性前列腺增生组织中明显增加;并且SRSF1和Survivin蛋白的阳性表达与前列腺癌Gleason评分呈正相关,同时前列腺癌组织中Survivin蛋白表达量的升高与SRSF1蛋白表达量的升高呈正相关,初步证明了SRSF1的作用可能是通过上调Survivin的表达,从而促进了前列腺癌的发生、发展。Objective To test the expression of serine/arginine rich splicing factor 1 （SRSF1） and apoptosis inhibiting factor（Survivin） in prostate cancer, and study their correlation with the pathological features of prostate cancer, so as to put forward the new targets in the treatment of prostate cancer. Methods SRSFI and Survivin protein was determined in 20 prostate tissue samples including prostate cancer （n =12） and benign prostat- ic hyperplasia （n =8 ） by immunohistochemical SP method. SRSF1 and SuIrvivin was correlated to pathological features, and both the relevance was analyzed （ no related reports at home and abroad）. Results The positive expression rate of SRSF 1 protein in prostate cancer tissue cells was 76.37± 5.06 %, which was significantly higher than that of benign prostatic hyperplasia （ 11.30%± 1.09%, P 〈 0.05 ） ; the positive expression rate of Survivin protein in prostate cancer tissue cells was 86.93%±3.21%, which was significantly higher than that of benign prostatic hyperplasia （ 17.67%±1.99%, P 〈 0.05 ） ; SRSF1 and Survivin protein expressed in prostate cancer organizations and were positively correlated to pathological Gleason grading, and there was significant correlation （P 〈 0.05 ）. Conclusion SRSF1 and Survivin protein were highly expressed in adenocareinoma tissue, which were significantly increased than that of benign hyperplasia of prostate tissue. The positive expression SRSF1 and Survivin protein were positively cor- related to pathological Gleason grading.The expression of Survivin protein was elevated with the expression of SRSF1 protein in prostate cancer. These preliminary evidence indicated that SRSF1 may up-regulate the expression of Survivin, and thus promote the occurrence and development of prostate cancer.

关 键 词：前列腺癌 良性前列腺增生 人丝氨酸/精氨酸富有剪接因子1 凋亡抑制因子 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]