基于酚类特征图谱的人参质量表征关联分析研究  

Quality characterization and correlation analysis based on phenolic characteristic spectrum of Panax ginseng

作　　者：陈唯[1] 杨元[1] 张芳[1] 李焕娟 孔静[1] 申立峰[2] 王亚利[1] 谭立[1] 赵博[1] 刘璐[1] 张元元[1] 姜艳艳[1,3] 石任兵[1,3] 

机构地区：[1]北京中医药大学中药学院国家中医药管理局中药经典名方有效物质发现重点实验室,100102 [2]首都医科大学中医药学院,100102 [3]北京市教委中药质量控制技术工程中心,100102

出　　处：《环球中医药》2016年第4期419-427,共9页Global Traditional Chinese Medicine

基　　金：国家“十二五”科技支撑计划(2012BAI29B06);北京中医药大学创新团队资助项目(2011-CXTD-12)

摘　　要：目的建立基于酚类特征图谱的人参质量表征关联分析方法与评价模式,为有效精准地评价人参质量奠定基础。方法运用HPLC-PDA法表征人参酚类特征图谱中13个特征峰及其所属化学类型,对16批人参饮片进行质的表征;建立HPLC同时测定对羟基苯甲酸、阿魏酸、槲皮素、山奈酚、异鼠李素含量的方法,并表征酚类成分和指标;建立可见分光光度法测定人参饮片中总酚的含量,基于酚类成分及其和指标与总酚的含量对16批人参饮片进行量的表征;对人参中各酚类成分及其和指标与总酚含量的关联性、质量表征规律进行分析,并基于参比饮片(批号16)对16批人参质与量的表征结果进行关联性分析。结果 16批人参酚类特征图谱中均含有13个特征峰,其中酚酸类10个,黄酮类3个;得出酚类有效指标性成分与总酚含量的相关系数范围及各成分含量分布规律,并得出各批次人参饮片中各成分含量与参比饮片比较的总体含量变化趋势及关联度。结论所建立的基于酚类特征图谱的人参质量表征关联分析模式,为关注人参质量整体关联性与应用有效性及有效精准地评价人参饮片质量奠定基础。Objective Based on phenolic constituents to establish the quality characterization and correlation analysis method of Panax ginseng,and to lay the foundation for evaluating the quality of ginseng. Methods HPLC-PDA method was used to characterize the 13 kinds of characteristic peaks and their chemical type of Panax ginseng,the quality of 16 batches of ginseng slices was characterized. The HPLC method was established to simultaneous detect the content of p-hydroxybenzoic acid,ferulic acid,quercetin,kaempferol and isorhamnetin,the content of the phenolics indicators were characterized,and visible spectrophotometry was established to detect the content of total phenols in Panax ginseng. The 16 batches of Panax ginseng＇s content of phenolic components,relevant indicators and total phenolic was characterized.And analysis the regularity of the relevance and quality characterization of each phenolic components and its indicators of ginseng with the total phenolic content,then correlatively analysis the results of quantity and quality of 16 batches of Panax ginseng based on reference piece（ No. 16）. Results In the 16 batch of phenolic compounds,13 characteristic peaks were found,including 10 phenolic acids and 3 flavonoids;The correlation coefficient range and the distribution law of the content of the total phenolic content and the effective index of phenols were found,and the change trend and correlation degree of the total content and the reference compound in each batch of ginseng were found. Conclusion The quality characterization and correlation analysis method of characteristic spectrum of Panax ginseng is based on phenolic constituents,it lays the foundation for the overall quality relevance and effective applicationand effective and accurate evaluation of the quality of ginseng pieces.

关 键 词：人参 特征图谱 质量评价 关联分析 酚酸类指标 黄酮类指标 总酚 

分 类 号：R284[医药卫生—中药学]