复发缓解型实验性变态反应性脑脊髓炎动物模型的建立  

Development of Relapsing- Remitting Experimental Autoimmune Encephalomyelitis Animal Model

作　　者：王静[1,2] 张旺倩[3] 褚楚[3] 吴守振[3] 王舒宁[3] 刘楠楠[4] 冯国栋[1] 

机构地区：[1]第四军医大学西京医院神经内科,陕西西安710032 [2]解放军第四五一医院神经内科,陕西西安710054 [3]第四军医大学药学院生物制药学教研室,陕西西安710032 [4]第四军医大学基础部教学实验中心,陕西西安710032

出　　处：《生物技术通讯》2016年第2期219-222,共4页Letters in Biotechnology

摘　　要：目的:建立复发缓解型实验性变态反应性脑脊髓炎(EAE)动物模型,进行病理学研究,为多发性硬化(MS)的发病机制研究及治疗药物研发提供合适的动物模型。方法:选择不同品系动物(昆明种、BALB/c和C57BL/6小鼠,SD大鼠),采用各自品系来源的脊髓和弗氏完全佐剂混合乳剂一次性注入动物双足垫,1周后半剂量注射进行一次加强,诱导EAE;观察发病情况,HE染色观察病理变化,监牢兰染色观察脱髓鞘情况。结果:昆明种、BALB/c和C57BL/6小鼠均无明显症状,HE染色小脑的脊髓无炎性细胞浸润;SD大鼠对照组正常,模型组临床症状发生率为90%以上,HE染色可见小脑白质及脊髓血管周围有大量的炎性细胞浸润,监牢兰染色可见大片髓鞘脱落。结论:EAE模型在昆明种、BALB/c及C57BL/6小鼠中用脊髓匀浆不易于诱导,而用SD大鼠脊髓髓鞘蛋白和弗氏完全佐剂混合乳剂可高效率诱导出同种SD大鼠的复发缓解型EAE。Objective： To develop the relapsing- remitting experimental allergic encephalomyelitis（EAE） animalmodel so that it can lay a foundation for molecular mechanism investigation and novel therapeutic drugs evaluationfor multiple sclerosis. Methods： Different strains of mice including Kunming, BALB/c, C57BL/6 mice and SD ratswere used to induce EAE models. The models were established by immunizing animals by spinal cord homogenateemulsified with complete Freund adjuvant（CFA） once and boosted one week later. The clinical manifestation wasobserved. Inflammatory cell infiltration and demyelination were detected by HE and fast blue stains, respectively.Results： No apparent syndrome can be found in mice in which rare infiltrating cells and demyelination was ob-served. As to SD rats, over 90% rats in model group, whereas none in control group, developed severe EAE. Alot of infiltrated inflammatory cells accompanied by severe demyelination were found around vessels in cerebellumand spinal cords. Conclusion： Relapsing-remitting EAE model can be effectively induced in SD rats by immuniza-tion with the homogenate of spinal cord and CFA.

关 键 词：多发性硬化 实验性变态反应性脑脊髓炎 动物模型 

分 类 号：R744.5[医药卫生—神经病学与精神病学]