连翘不同部位总酚含量测定及抗氧化活性比较研究  

Study on comparison of antioxidant activity and determination of total phenol content in different parts of Forsythia suspensa

作　　者：程启斌[1,2] 李石飞[1] 张立伟[1] 

机构地区：[1]山西大学分子科学研究所,山西太原030006 [2]山西大学化学化工学院,山西太原030006

出　　处：《化学研究与应用》2016年第5期610-616,共7页Chemical Research and Application

基　　金：科技部科技支撑计划项目(2011BAI07B06)资助;山西省国际合作项目(2014081049-1)资助;山西省科学技术发展计划项目(2014ZDO0402)资助

摘　　要：以山西产连翘为实验原料,采用单因素试验考察法优化了超声辅助提取连翘中总酚的最佳条件,即70%甲醇超声提取2次,10 min/次,料液比1∶20,此方法提取方便快捷,效率较高,为连翘总酚提取工艺提供一定参考价值。另外,在此条件下采用Folin-Ciocalteu法分别测定了老翘、青翘、连翘叶及连翘花中总酚含量,结果显示连翘叶中含量最高,花及果实次之;采用1,1-二苯基-2-苦肼基(DPPH·)清除法综合考察其抗氧化活性,结果与总酚含量顺序一致,二者之间呈明显正相关性,对于连翘资源的综合开发利用以及连翘中天然抗氧化剂的研究提供了重要的科学依据和指导作用。Shanxi Forsythia suspensa as experimental material,the single factor experiment was used to optimize the ultrasonic assis-ted extraction conditions of total phenol in Forsythia suspensa, the determined optimum technological conditions that is 20 times the amount of 70%ethanol,extracted two times with 10 min each time. This extraction process is convenient and high efficiency,that provide certain reference value for the total phenol extraction process of Forsythia suspense. In addition, the content of total phenol thatin fructus,leaves and flowers from Forsythia suspensa were measured by using Folin-Ciocalteu assayunder that condition,the re-sults showed that the content in leaves is higher than fruit and flowers. 1,1-Diphenyl-2-picrylhydrazyl free radical? (DPPH·)-scav-enging test was used to measure the antioxidant activity,the results were relevant to the content of total phenol,and has shown sig-nificantly positive correlation. The research will provides important scientific basis and guidance for comprehensive development of Forsythia suspensa resource utilization and the research about natural antioxidant that in Forsythia suspense.

关 键 词：连翘 总酚 FOLIN-CIOCALTEU法 超声提取 抗氧化 

分 类 号：O657.3[理学—分析化学]