基于EST-SSR标记的蓝莓品种指纹图谱构建及遗传多样性  

Fingerprinting Construction and Genetic Relationship Analysis of Blueberry Based on EST-SSR Markers

作　　者：高雄梅 徐国辉[2] 王贺新[2] 侯义龙[1] 

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]大连大学现代农业研究院,辽宁大连116622

出　　处：《贵州农业科学》2016年第4期110-115,共6页Guizhou Agricultural Sciences

基　　金：辽宁省博士科研启动基金项目"高丛蓝莓自然杂交优良品系的筛选及其DNA指纹鉴定"(20141120);辽宁省科技计划项目"蓝莓树莓种质资源创新及优质高效栽培技术研究"(2013204001);蓝莓优良新品种的选育及推广应用(XDNY-NYKJ-2015-001);大连金州国家农业科技园区科技计划项目"蓝莓优良品种高效栽培技术示范"(2014-004);昆明市科技计划项目"蓝莓品种选育及关键栽培技术创新"(2014-04-A-N-01-3099)

摘　　要：为给蓝莓品种鉴别、优良杂交组合选配提供分子水平依据,从40对EST-SSR引物中筛选多态性良好的引物组合,选取其中引物CA231构建北高丛蓝莓栽培种的DNA指纹图谱、数字指纹图谱,利用非加权类平均法进行遗传多样性分析。结果表明：10个引物对组合共扩增出206个位点,其中多态性位点203个,占98.12%。每条引物的多态性位点数为9~48个,平均每条引物扩增出20.6个位点,平均多态性位点为20.3个。引物CA231扩增位点数最多,多态性比率为100%,且可以鉴别所有试验材料。构建30个北高丛蓝莓栽培品种的指纹图谱,并对其亲缘关系分析表明,在遗传相似系数为0.52时,30个品种聚为一类,当遗传相似系数为0.63时,77.7%的品种仍聚为一类。To provide basis for cultivar identification and apolegamy of excellent hybridization combination at the molecular level,EST-SSR markers were employed to analyze on fingerprinting and genetic diversity of north highbush blueberry cultivars.Ten of 40 pairs of EST-SSR primers were screened out based on five cultivars which were selected randomly.The 10 primer pairs amplified a total of 206alleles（including 203 polymorphic alleles）among the 30 cultivars,and the ratio of polymorphism was as high as 98.12%.Alleles amplified by each pair of primers ranged from 9to 48,with a mean of 20.6,and the mean of polymorphism alleles for each pair was 20.3.30 cultivars were univocally identified using only one EST-SSR primer pair（CA231）,which one＇s alleles was the most and the ratio of polymorphism was100%.UPGMA cluster analysis of genetic relationship between cultivars showed that all the materials were clustered into one group at the genetic similarity coefficient of 0.52,and 77.7% of the cultivars were still clustered together at the genetic similarity coefficient of 0.63.

关 键 词：蓝莓 EST-SSR 指纹图谱 遗传多样性 

分 类 号：S663.9[农业科学—果树学]