沉默URG11基因对前列腺癌LNCaP细胞增殖、迁移与侵袭的影响  

Effect of URG11 gene silencing on proliferation and invasion abilities of human prostate cancer cells

作　　者：潘斌[1] 邓志海[1] 吴友科 梁蔚波[1] 曾传蓉 刘海平 

机构地区：[1]暨南大学附属第一医院泌尿外科,广东广州510632 [2]连平县第二人民医院外科,广东河源517139

出　　处：《中国病理生理杂志》2016年第4期658-664,共7页Chinese Journal of Pathophysiology

基　　金：广东省医学科学技术研究基金资助项目(No.A2015557);中央高校基本科研业务费专项资金资助项目(No.21613317)

摘　　要：目的:观察上调基因11(up-regulated gene 11,URG11)在前列腺癌细胞系中的表达及降低URG11表达对人前列腺癌LNCa P细胞增殖和侵袭能力的影响。方法:用real-time PCR和Western blot检测前列腺癌细胞系和正常前列腺上皮细胞系中URG11 mRNA和蛋白水平;设计针对URG11基因的siRNA靶序列,转染LNCa P细胞,采用流式细胞仪检测细胞周期和凋亡,MTS测定LNCa P细胞增殖能力,划痕和侵袭实验评价LNCa P细胞迁移及侵袭能力。结果:在LNCa P、DU145、PC3前列腺癌细胞系和RWPE-1正常前列腺上皮细胞系中URG11 mRNA和蛋白表达水平存在显著差异,在前列腺癌细胞中URG11 mRNA和蛋白表达水平明显升高(P<0.05)。与对照组相比,转染URG11 siRNA的LNCa P细胞增殖停滞在G_1/S期并诱导前列腺癌细胞凋亡,转染组的细胞凋亡率为8.79%±0.12%,而且LNCa P肿瘤细胞的迁移及侵袭能力明显下降(P<0.05)。结论:URG11在前列腺癌系中高表达。通过RNAi沉默URG11基因能明显抑制LNCa P细胞增殖和侵袭能力,并诱导细胞凋亡。AIM： To investigate the expression of up-regulated gene 11（ URG11） in prostate cancer cell line and the effect of URG11 si NRA on the proliferation and invasion of human prostate cancer LNCa P cells. METHODS： The mRNA and protein levels of URG11 in prostate cancer cell lines and normal prostate epithelial cell line were evaluated by real-time PCR and Western blot. LNCa P cells were transfected with designed siRNA using the liposome method. The proliferation,apoptosis,migration and invasion abilities of the LNCa P cells were evaluated by MTS assay,flow cytometry,wound-healing assay and Transwell assay. RESULTS： The expression of URG11 at mRNA and protein levels in the DU145,PC3,LNCa P cell lines was significantly higher than that in RWPE-1 cell line. Compared with the control group,the proliferation of LNCa P cells with URG11 siRNA was stagnant in G1/ S phase and induced apoptosis. The proliferation of LNCa P cells at 0 h,24 h,48 h and 72 h was inhibited after URG11 expression was down-regulated（ P 0. 05）. Transwell assay showed that migration（ P 0. 05） and invasion（ P 0. 05） were also inhibited. CONCLUSION： URG11 is highly expressed in prostate cancer. Silencing of URG11 significantly inhibits the proliferation and invasion and induces apoptosis of LNCa P cells.

关 键 词：上凋基因11 前列腺癌 RNA干扰 肿瘤侵袭 

分 类 号：R730.23[医药卫生—肿瘤]