mir-99a的表达与上皮性卵巢癌患者预后的关系  

Relationship between mir-99a expression and prognosis of epithelial ovarian cancer

作　　者：徐志宏[1] 吴小华 姚铁柱[3] 王晓翔[1] 段晓阳[1] 史健[1] 

机构地区：[1]河北医科大学第四医院肿瘤内科,河北石家庄050011 [2]白求恩国际和平医院妇产科,河北石家庄050000 [3]河北医科大学第四医院心内科,河北石家庄050011

出　　处：《山东大学学报（医学版）》2016年第3期77-80,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨mir-99a在上皮性卵巢癌(EOC)患者中的表达与临床病理特征及预后的关系。方法采用实时荧光定量PCR(qRT-PCR)法检测137例EOC患者组织中mir-99a的表达水平,分析mir-99a表达与卵巢癌患者的临床病理资料的相关性。采用Kaplan-Meier法进行生存分析并绘制生存曲线,生存因素采用log-rank检验。结果 EOC患者组织中mir-99a的表达较癌旁正常组织降低(P<0.001)。mir-99a的低表达与高组织学分级(P=0.027)、淋巴结转移(P=0.001)、腹膜转移(P<0.001)和较高的FIGO分期(P<0.001)有关。Kaplan-Meier分析与log-rank检验结果显示,mir-99a的低表达与EOC患者生存期短有关(对数秩检验,P=0.005)。多因素分析结果显示,mir-99a表达是患者总体生存的独立预测因子(HR为2.368,95%CI:1.621,10.925,P=0.016)。结论mir-99a的表达是上皮性卵巢癌患者独立的预后因素,mir-99a可能成为临床实践中一项新的诊断和预后标志物。Objective To investigate the mir-99 a expression in epithelial ovarian cancer（ EOC）,and its relationship with the clinicopathological features and prognosis. Methods The mir-99 a expression in 137 cases of EOC tissues was detected with qRT-PCR assay. The correlation between mir-99 a expression and clinicopathological features of EOC were analyzed. Survival curves for patients with different mir-99 a expressions were plotted using the Kaplan-Meier method and compared using the log-rank test. Results The mir-99 a expression reduced significantly in EOC tissues compared with that in adjacent normal tissues（ P〈0. 001）. Lowmir-99 a expression was significantly correlated with higher histological grade（ P = 0. 027）,lymph node metastasis（ P = 0. 001）,peritoneal metastasis（ P〈0. 001）,and higher FIGO stage（ P〈0. 001）. Kaplan-Meier analysis with log-rank test revealed that lowmir-99 a expression was correlated with short survival time of patients with EOC（ log-rank test,P = 0. 005）. Multivariate analysis showed that mir-99 a expression was an independent prognostic factor for the overall survival（ HR： 2. 368,95% CI： 1. 621-10. 925,P =0. 016）. Conclusion The mir-99 a expression is an independent prognostic factor of patients with EOC. It can serve as a diagnostic and prognostic marker in clinical practice.

关 键 词：mir-99a 上皮性卵巢癌 临床病理学 预后 

分 类 号：R737.31[医药卫生—肿瘤]