荧光原位杂交技术检测胃癌组织中人表皮生长因子受体2基因及其与P53蛋白表达的相关性  

FISH technology applied to detect HER2 gene in gastric cancer tissue of patients with gastric cancer and its correlation with expression of P53 protein

作　　者：徐燕[1] 蔡媛[1] 荆娟[1] 马宁[1] 张亚妮[1] 赵斌[1] 

机构地区：[1]陕西省宝鸡市中心医院病理科,721008

出　　处：《山西医药杂志》2016年第8期882-885,共4页Shanxi Medical Journal

摘　　要：目的探讨应用荧光原位杂交(FISH)技术检测胃癌组织中人表皮生长因子受体(HER2)基因及其与P53蛋白表达的相关性。方法选取2012年1月2015年1月于我院治疗的50例胃癌患者为研究对象,采用FISH技术和免疫组织化学技术检测患者胃癌组织中HER2基因与P53蛋白状态,观察HER2基因、P53蛋白与临床病理的关系。结果 50例胃癌组织中有14例HER2基因扩增,26例HER2蛋白表达;HER2蛋白表达呈阳性与强阳性患者中HER2基因扩增率高于HER2蛋白表达呈弱阳性患者HER2基因扩增率(P<0.05);27例胃癌患者P53蛋白表达,且胃癌浸润深度越深患者或伴有淋巴结转移患者,其HER2基因扩增率与P53蛋白表达率明显高于胃癌浸润较浅或无淋巴结转移者(P<0.05)。结论胃癌组织中的HER2基因的扩增与P53蛋白的表达呈正相关,且胃癌浸润深度越深或伴有淋巴结转移患者的HER2基因扩增率与P53蛋白表达率越高,因此,HER2基因扩增与P53蛋白表达情况可作为评定患者病情与预后的有效指标。Objective To investigate FISH technology applied on patients with gastric cancer to detect HER2 gene in gastric cancer tissue and its correlation with the expression of P53 protein.Methods Fifty patients with gastric cancer treated in our hospital from January,2012 to January,2015 were selected as the research subjects.FISH technique and immunohistochemical technique were applied to detect the status of HER2 gene and P53 protein in gastric cancer tissues of patients,and the correlations among HER2 gene,P53protein and clinical pathology were observed.Results Among 50 patients with gastric cancer,there were 14 patients with HER2 gene amplification in gastric cancer tissues and 26 patients with HER2 protein expression.HER2 gene amplification rate of patients with positive and strong positive HER2 protein expression was higher than that of patients with weakly positive HER2 gene expression（P〈0.05）.The rates of HER2 gene amplification and P53 protein expression of patients with gastric carcinoma,deeper infiltration depth of gastric cancer or lymph node metastasis were significantly higher than those of patients with superficial infiltration of gastric cancer or without lymph node metastasis（P〈0.05）.Conclusion The amplification of HER2 genes in gastric cancer tissues of patients with gastric cancer were positively correlated to the expression of P53 protein,and the deeper the infiltration depth of gastric cancer in the patients or in patients with lymph node metastasis,the higher the HER2 gene amplification rate and the P53 protein expression rate of P53 protein expression are.Therefore,HER2 gene amplification and P53 protein expression condition may be used as an effective index to evaluate illness state and prognosis of patients.

关 键 词：胃肿瘤 基因 ERBB-2 基因 p53 

分 类 号：R735.2[医药卫生—肿瘤]