口腔扁平苔藓病灶中Toll样受体4与TNF-α的表达  

The expression of Toll like receptor 4 and TNF-α signal pathway in oral lichen planus lesion

作　　者：王学莹[1] 宋典坤[1] 

机构地区：[1]福建医科大学附属协和医院,福建福州350001

出　　处：《实用医药杂志》2016年第4期331-334,共4页Practical Journal of Medicine & Pharmacy

基　　金：福建省自然科学基金面上项目(2013J01314)

摘　　要：目的探讨TLR4、TNF-α在口腔扁平苔藓(OLP)发病中的可能作用。方法采用免疫组化法检测OLP组与正常对照组口腔黏膜蜡块标本中TLR4、TNF-a的表达;采用q RT-PCR法检测TLR4的基因表达。结果OLP组中的TLR4、TNF-α蛋白及TLR4 mRNA表达均显著高于对照组(P<0.05);TNF-α蛋白与TLR4蛋白表达呈正相关(P<0.05)。结论 OLP病灶部位TLR4 mRNA表达激活,TLR4、TNF-α表达增多,与OLP局部炎症和破坏的迁延不愈有关。Objective To investigate the possible role of TLR4 and TNF-α in OLP. Methods Immunohistochemistry was used to detect the expression of TLR4,TNF-α in the oral mocusa of patients with OLP and normal oral mucosa（control group）,respectively. Quantitative real-time PCR was used to detect the expression of m RNA of TLR4. Results The IHC expression of TLR4,TNF-α in OLP was significantly stronger than those in control group（P〈0.05）,TLR4 m RNA expression did so. There was positive correlation between the expression of TNF-α and TLR4（P〈0.05）. Conclusion The high expression and activation of TLR4 and increased expression of TNF-α may have some relation to long-lasting inflammation and destruction in the lesion of OLP.

关 键 词：口腔扁平苔藓 TOLL样受体4 肿瘤坏死因子Α 

分 类 号：R781.31[医药卫生—口腔医学]