力竭运动后不同时相大鼠心电图、心功能变化及Nrf2的作用  

The effect of nuclear factor erythroid-2-related factor2 on the changes of cardiac function and electrocardiogram in rats after exhausted exercise

作　　者：徐鹏[1] 康亭 刘海燕[1] 孙卫卫[1] 解亚楠[1] 曹雪滨[1] 

机构地区：[1]解放军第252医院心内科,河北保定071000 [2]保定市第二医院急诊科,河北保定071000

出　　处：《中国应用生理学杂志》2016年第2期146-151,共6页Chinese Journal of Applied Physiology

基　　金：全军医学科技"十二五"重点项目(13WS11J058);全军医学科研"十二五"面上项目(CWS12J064);后勤科研面上项目(CBJ13J006)

摘　　要：目的:研究大鼠力竭运动及运动结束后心电图、心功能的动态变化规律及转录因子E2相关因子(Nrf2)相关的氧化应激变化,为运动性心脏损伤防治提供依据。方法:SD大鼠随机分为5组(n=6):对照组(Con)组、力竭组(EE)、力竭恢复6 h,12 h,24 h组(EER6、EER12、EER24组)。急性力竭游泳建立损伤模型。分别对各组动物进行心电图描记,压力容积导管检测心功能改变,ELISA法观测血清ROS,Nrf2,GPX及CAT变化。结果:1 EE组心率(HR),收缩末期压力(Pes),发展压,动脉弹性,压力上升,下降最大速率(dP/dt_(max)、-dP/dt_(min))降至最低。舒张末期压力容积、收缩末期容积、搏出量、Tau值增大。EER6、EER12、EER24组HR、Pes、dP/dt_(max)、-dP/dt_(min)与EE组相比均差异显著。2EE组、EER6、EER12、EER24组与Con组相比心率加快,QT间期延长,P波R波ST段数值增高,但恢复各组与EE组相比无统计学意义。3EE组大鼠血清ROS、Nrf2含量升高,GPX含量降低,CAT在EER6组降至最低。4血清Nrf2水平与ROS,-dP/dt_(min)呈正相关,与HR、Ea呈负相关。血清ROS水平与EF,-dP/dt_(min)呈正相关,与HR、Ea、dP/dt_(max)呈负相关。结论:力竭运动后心脏生物电改变,舒缩功能均受损,以舒张功能减退突出,随力竭恢复时间延长,心脏舒缩功能逐步恢复,这与Nrf2调节GPX,CAT降低氧化应激有关。Objective： In order to provide the experimental basis for the prevention of exercise-induced cardiac injury, we evaluated the effects of nuclear factor erythroid-2-related factor2（Nrf2） on the changes of cardiac function and electrocardiogram in rats after exhaustive exercise. Methods： SD rats were randomly divided into 5 groups （ n = 6） ： control group （Con）, exhaustied exercise group （EE）, 6h, 12 h, 24 h recovery from exhaustied exercise group（EER6 EER12 EER24）. The animal models of exercise-induced myocardial injury were established according to Thomas＇ method. Rats were forced to swim until they were exhausted. The electrocardiogranls were recorded in conscious rats. Cardiac function of rats was recorded and analyzed by Millar pressure-volume system. The changes of catalase（CAT）, glutathione peroxidase （GPX）, Nrf2 and reactive oxygen speies（ROS） were detected by ELISA, respectively. Results： （1）Compared with the control group and recovery groups（ EER6, EER12, and EER24）, the heart rate （HR）, left ventricular end systolic pressure （Pes）, arterial elasticity （Ea）, the maximum rate of left ventricular pressure rise （dP/dtmax ）, peak rate of left ventricular pressure decline （-dP/dtmin） and left ventricular end diastolic pressure volume relationship curve slope （ESPVR） in the EE group decreased significantly, while left ventricular end diastolic volume （Ved）, Pes, left ventrieular end systolic volume （Ves）, stroke volume, and Tan value increased significantly. Besides, HR, Pes, dP/dt max, -dP/dt min in recovery groups were significantly different with those in EE group, but there had no difference with those in the Con group. （2） Compared with the control group, heart rate was increased, QT intervals were prolonged P wave, R wave and ST segments were increased in EE and recovery groups, but the changes of above-mentioned indexes in recovery groups had no statistical significant difference with those in EE group. （3） Compare

关 键 词：心功能 力竭运动 氧化应激 转录因子E2相关因子 大鼠 

分 类 号：R-332[医药卫生] R331.3