福州地区大黄鱼变形假单胞菌的分离与鉴定  

Isolation and identification of Pseudomonas plecoglossicida in large yellow croaker(Pseudosciaena crocea) of Fuzhou area

作　　者：刘年锋[1] 郭睿[1] 江小斌[1] 张善霹[1] 王伟[1] 郑晨晖[1] 杨小强[1] 

机构地区：[1]福州市海洋与渔业技术中心,福建福州350026

出　　处：《渔业研究》2016年第1期14-20,共7页Journal of Fisheries Research

基　　金：农业部2013年国家水生动物疫情监测计划--福建省刺激隐核虫病监测;福州市海洋与渔业局2013年水产养殖病害监测专项

摘　　要：为研究2013年春夏之际罗源湾海区网箱养殖大黄鱼(Pseudosciaena crocea)内脏出现白点症状的病原,从具有临床症状的大黄鱼内脏中分离到优势菌,经回接感染试验证实该菌为病原菌;分析该病原菌的形态特征、理化特性、分类地位及药物敏感性等特性,结合16S r DNA序列构建的进化树与假单胞菌属恶臭群中的变形假单胞菌同源性最高,在99%以上,并通过看家基因gyr B序列分析进一步验证该菌为变形假单胞菌(Pseudomonas plecoglossicida);该菌对强力霉素、诺氟沙星、恩诺沙星3种抗生素敏感,对奥氟沙星、左氟沙星、四环素3种抗生素中度敏感。The cultured large yellow croakers（Pseudosciaena crocea） of Luoyuan Bay got white nodules disease at the end of spring and the beginning of summer in 2013.The pathogen was isolated from spleen,liver and kidney with significant pathological signs of fishes,then its morphological,physiological and biochemical characteristics,taxonomic status,and drug sensitivity were analyzed.The 16 S r DNA sequences of the pathogen was more than 99% homology with Pseudomonas plecoglossicida in P.putida group,the analysis of housekeeping gene gyr B confirmed its phylogenetic affiliation and showed identities more than 99% homology,which was also demonstrated by biochemical tests.The test result of the sensitivity to antibiotics showed that doxycycline,norfloxacin and enrofloxacin had the most inhibitive effects on the strains,while ofloxacins,levofloxacin and tetracycline had intermediate sensitive.

关 键 词：大黄鱼 变形假单胞菌 16S RDNA GYRB 

分 类 号：S941[农业科学—水产养殖]