IL-6对肝癌细胞增殖、侵袭和转移的影响  

Effect of IL-6 on proliferation, invasion and metastasis in liver cancer cell

作　　者：李丹[1] 叶丽平[1] 仇会会[1] 宋佳[1] 

机构地区：[1]辽宁医学院基础医学院病理生理学教研室,辽宁锦州121001

出　　处：《解放军医学院学报》2016年第4期377-380,385,共5页Academic Journal of Chinese PLA Medical School

基　　金：辽宁省自然科学基金项目(2015020362)~~

摘　　要：目的观察白细胞介素-6(interleukin-6,IL-6)对肝癌SMMC-7721细胞增殖、侵袭和转移的影响并探究其作用机制。方法体外培养SMMC-7721细胞,分别加入不同浓度IL-6(0、5、10、20、40 ng/ml)和(或)AKT抑制剂LY294002。MTT、细胞划痕实验、Transwell侵袭实验、细胞黏附实验分别检测细胞的增殖、转移和侵袭能力的变化;Western blot方法检测AKT、p-AKT、MMP-9、CD44、Ezrin蛋白的表达量。结果 IL-6组(5、10、20 ng/ml)浓度依赖性促进细胞增殖率、划痕愈合率、侵袭细胞数目和黏附细胞吸光度值增加(P<0.05);IL-6浓度20 ng/ml与40 ng/ml之间差异无统计学意义(P>0.05)。IL-6最佳作用浓度组(20 ng/ml)细胞增殖率(62.76%±6.86%)、划痕愈合率(92.37%±9.38%)、侵袭细胞数目(174.41±15.14)、黏附细胞吸光度值(0.531±0.055),P-AKT、MMP-9、CD44、Ezrin蛋白的表达量均高于对照组和IL-6(20ng/ml)+LY296002(20μmol/L)组(P<0.05)。各组AKT蛋白表达量差异无统计学意义(P>0.05)。结论 IL-6可能通过AKT通路影响MMP-9、CD44、Ezrin蛋白的表达,促进肝癌细胞的增殖、侵袭和转移。Objective To observe the influence of interleukin-6（IL-6） on liver cancer SMMC-7721 cells’ proliferation, invasion and metastasis, and explore its mechanism. Methods SMMC-7721 cells were cultured in vitro, IL-6（0, 5, 10, 20, 40 ng/ml） of different concentrations and/or AKT inhibitor LY294002 were added. MTT, wound healing assay, Transwell invasion assay, cell adhesion assay were used to detect changes in cell proliferation, metastasis and invasion; Western blot method was used to detect the expression quantity of AKT, p-AKT, MMP-9, CD44, and Ezrin protein. Results The IL- 6（5, 10, 20 ng/ml） promoted the cell proliferation rate, the healing rate of scratches, the number of invading cells and adherent cell absorbance value（P 〈0.05） concentration- dependently; and there was no statistically significant difference between concentration of 20 ng/ml and 40 ng/ml of IL- 6（P 〉0.05）. The cell proliferation rate（62.76%±6.86%）, healing rate of scratches（92.37%±9.38%）, number of invading cells（174.41±15.14）, adherent cell absorbance value（0.531±0.055）, and the protein expression of P- AKT, MMP- 9, CD44 and Ezrin of IL- 6 optimal effect concentration group（20 ng/ml） were higher than those of control group and IL-6（20 ng/ml） ＋ LY296002（20 ？mol/L） group（P 〈0.05）. There was no statistical significance in AKT protein expression between each group（P 〉0.05）. Conclusion IL-6 can influence the expression of MMP-9, CD44, Ezrin protein by AKT pathway, and promote proliferation, invasion and metastasis of liver cancer cell.

关 键 词：白细胞介素-6 蛋白激酶B 基质金属蛋白酶9 归巢细胞黏附分子 埃兹蛋白 

分 类 号：R735.7[医药卫生—肿瘤]