自分泌运动因子AMF对人胶质母细胞瘤U251细胞迁移、侵袭的影响及相关机制研究  

Effects of Autocrine Motility Factor(AMF) on the Migration and Invasion of Glioblastoma U251 Cells and Their Mechanism

作　　者：李阳[1] 汤宁[2] 刘哲宇[3] 孙铮[3] 

机构地区：[1]大连医科大学附属第一医院神经外科,大连116001 [2]大连医科大学药学院,大连116044 [3]大连医科大学中西医结合基础研究所,大连116044

出　　处：《中国细胞生物学学报》2016年第4期369-374,共6页Chinese Journal of Cell Biology

基　　金：中国博士后科学基金面上项目(批准号:2013M541232)资助的课题~~

摘　　要：为了探讨自分泌运动因子(autocrine motility factor,AMF)对人胶质母细胞瘤U251细胞迁移、侵袭影响及其相关分子机制,该实验采用了RT-PCR及免疫印迹法检测RNA干扰AMF后U251细胞中AMF的表达变化;细胞划痕实验、Transwell实验分别观察了AMF干扰前后U251细胞迁移、侵袭能力的变化;免疫印记检测AMF干扰前后细胞中总Akt、p-Akt、Sox2、基质金属蛋白酶-2(matrix metalloprotein-2,MMP-2)及MMP-9蛋白水平的变化。研究结果表明,AMF成功干扰后U251细胞的迁移和侵袭能力受到抑制,p-Akt、Sox2、MMP-2和MMP-9蛋白表达水平降低。该研究表明,AMF敲低可以通过下调PI3K/Akt信号通路活性及Sox2、MMP-2和MMP-9蛋白水平,抑制人胶质母细胞瘤U251细胞迁移和侵袭。To explore the effects of autocrine motility factor（AMF） on migration and invasion of U251 cells and their related mechanisms, the expression of AMF was interfered by si RNA. The expression of AMF in U251 cells was detected by RT-PCR and Western blot. Wound healing assay and transwell invasion were used to detect the migration and invasion ability of U251 cells. Western blot was used to detect the protein levels of total Akt, p-Akt, Sox2, MMP-2 and MMP-9 before and after down regulation of AMF. Our results showed that the migration and invasion ability of U251 cells, which AMF was down-regulated successfully, has been significantly inhibited. Protein levels of phosphorylation Akt, Sox2, MMP-2 and MMP-9 were decreased significantly compared with the control group. These results indicated that AMF has played an important role in inhibiting the migration and invasion of U251 cells, through down-regulation the expression of PI3K/Akt pathway, Sox2, MMP-2 and MMP-9 proteins.

关 键 词：胶质母细胞瘤 AMF 迁移 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]