miR-100表达与乳腺癌患者淋巴结转移及FZD-8表达的相关性研究  

The correlation of miR-100 expression with lymph node metastasis and FZD-8 protein expression in patients with breast cancer

作　　者：于兆进[1] 江茜[1] 韩丽[1] 关舒[2] 颜媛媛[1] 魏敏杰[1] 何苗[1] 

机构地区：[1]中国医科大学药学院药理教研室,110122 [2]中国医科大学附属第一临床医院乳腺外科

出　　处：《天津医药》2016年第4期394-396,共3页Tianjin Medical Journal

基　　金：辽宁省高校创新团队支持计划资助项目(LT2014016);沈阳市科技计划项目(F14-232-6-05)

摘　　要：目的考察临床乳腺癌病理组织中mi R-100和Wnt通路关键因子卷曲蛋白(FZD)-8表达之间的关系,并分析mi R-100表达与乳腺癌患者淋巴结转移的关系。方法选取中国医科大学附属第一医院乳腺外科术后的50例乳腺癌患者,通过原位杂交检测乳腺癌患者病理组织及其配对的癌旁组织中mi R-100的表达;免疫组化检测2种组织中FZD-8的表达;比较有无淋巴结转移患者癌组织中mi R-100表达情况,分析乳腺癌组织mi R-100与FZD-8表达的相关性。结果 mi R-100在乳腺癌组织中的表达明显低于其配对的癌旁组织[2.00(1.00,3.00)vs.6.00(3.50,8.00)];有淋巴结转移者癌组织中mi R-100低表达[1.50(1.00,2.75)vs.3.00(2.00,4.00)];与癌旁组织相比,FZD-8在乳腺癌组织中高表达[8.00(6.00,9.00)vs.6.00(3.75,9.00)],且其表达水平与mi R-100的表达呈负相关(rs=-0.592,P<0.001)。结论 mi R-100作为抑癌mi RNA参与乳腺癌转移的调节,可能与其调控FZD-8的表达有关。Objective To evaluate the expression and relationship of mi R-100 and FZD-8, one of the major compo-nents of Wnt signaling pathway, and the correlation of their expressions with lymph node metastasis in patients with breastcancer. Methods The expression of mi R-100 was determined in 50 samples of human breast cancer tissues and adjacentnormal tissues by in situ hybridization. The correlation of mi R-100 expression with lymph node metastasis was analyzed byMann-Whitney U test. The expression of FZD-8 was measured in 50 samples of human breast cancer tissues and adjacentnormal tissues by immunohistochemistry. The correlation of the mi R-100 expression with the protein expression of FZD-8was evaluated by Pearson rank analysis. Results The expression of mi R-100 was significantly lower in human breast can-cer tissues than that in adjacent normal breast tissues [2.00（1.00, 3.00） vs. 6.00（3.50, 8.00）]. The mi R-100 expression waslower in patients with lymph node metastasis than that in patients without lymph node metastasis [1.50（1.00, 2.75） vs. 3.00（2.00, 4.00）]. The expression of FZD-8 was significantly higher in human breast cancer tissues than that in adjacent normalbreast tissues [8.00（6.00, 9.00） vs. 6.00（3.75, 9.00）]. The mi R-100 expression was negatively correlated with the FZD-8 pro-tein expression in human breast cancer tissues（rs=-0.592, P 0.001）. Conclusion The mi R-100, as an anti-metastasis-mi RNA, may involve in the metastasis of breast cancer, which may be related with the regulation of the expression of FZD-8.

关 键 词：乳腺肿瘤 淋巴转移 WNT蛋白质类 微RNAS miR-100 WNT通路 卷曲蛋白8 

分 类 号：R737.9[医药卫生—肿瘤]