密度梯度离心联合上皮细胞黏附分子免疫磁珠富集法检测卵巢癌循环肿瘤细胞及其与肿瘤复发转移的相关性  

Circulating tumor cells of ovarian cancer detected by density gradient centrifugation in combination with epithelial cell adhesion molecule immunomagnetic enrichment and its relationship with tumor recurrence and metastasis

作　　者：韩晓芳[1] 王晓成[2] 王蔼明 

机构地区：[1]山西省人民医院生殖科,太原030012 [2]山西省人民医院病案室,太原030012 [3]北京海军总医院生殖科

出　　处：《中国药物与临床》2016年第4期460-462,共3页Chinese Remedies & Clinics

基　　金："十二五"国家科技支撑计划(2012BAI32B05)

摘　　要：目的检测卵巢癌患者外周血中循环肿瘤细胞(CTCs)与肿瘤复发转移的相关性。方法采集50例卵巢癌患者的外周血,应用密度梯度离心联合上皮细胞黏附分子免疫磁珠富集法检测外周血中的CTCs的数目,并比较其与患者临床特征之间的关系。结果 50例卵巢癌患者中32例为CTCs阳性,CTCs阳性与远处转移(P<0.05)和复发密切相关(P<0.05),而与性别、年龄、肿瘤部位、TNM分期、肿瘤分化程度及淋巴转移无关(P>0.05)。结论 CTCs可以预测卵巢癌远处转移和复发。Objective To determine the relationship between circulating tumor cells（CTCs） of ovarian cancer in the peripheral blood and tumor recurrence and metastasis. Methods Peripheral blood samples were collected from50 ovarian cancer patients. Density gradient centrifugation in combination with epithelial cell adhesion molecule im-munomagnetic enrichment was used to determine the number of CTCs in the peripheral blood. The findings were compared with clinical characteristics of the patients. Results Of 50 patients with ovarian cancer, 32 were CTC-positive. Positive CTCs were closely correlated with distant metastasis（P〈0.05） and tumor recurrence（P〈0.05）, but not with gender, age, tumor location, TNM stage, tumor differentiation and lymphatic metastasis（all P〉0.05）. Conclusion CTCs may predict metastasis and recurrenceof ovarian cancer.

关 键 词：卵巢肿瘤 肿瘤细胞 循环 密度梯度离心 免疫磁珠富集法 

分 类 号：R737.31[医药卫生—肿瘤]