Screening and Genetic Analysis of the Hydrogen Peroxide Sensitive Mutant hps12 of Arabidopsis thaliana  

拟南芥响应过氧化氢突变体的筛选及遗传分析（英文）

作　　者：雷凯健[1,2] 王棚涛[1] 刘浩[1] 

机构地区：[1]河南大学生命科学学院植物逆境生物学重点实验室,河南开封475004 [2]河南大学药学院,河南开封475004

出　　处：《Agricultural Science & Technology》2016年第4期797-800,共4页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31170253);Key Scientific Research Foundation of the Higher Education Institutions of Henan Province,China(15A180001)~~

摘　　要：In order to investigate the mechanism of ROS signaling transduction in after-germination process, we screened a hydrogen peroxide sensitive mutant from EMS mutant library of Arabidopsis thaliana. Hydrogen peroxide sensitive 12（hps12）was screened from this library. Phenotype analysis indicated that hps12 had dwarf plant and shorter pod than wild type（WT）. The cotyledon greening rate of hps12 was decreased after being treated with 4 mmol/L H_2O_2. Furthermore, the isolated leaves of hps12 mutants showed more obvious symptoms of senescence than WT under the treatment of 10 mmol/L H_2O_2. Genetic analysis suggested that hps12 was a monogenic recessive mutant.利用甲基磺酸乙酯(EMS)诱变获得的拟南芥突变体库筛选过氧化氢(H_2O_2)敏感突变体,以期为研究氧化胁迫的分子机制提供遗传材料。从该突变体库中筛选出一株H_2O_2敏感突变体hps12(hydrogen peroxide sensitive 12)。表型分析发现,hps12突变体植株矮小,果荚较短,并且在4 mmol/L H_2O_2处理下hps12的子叶变绿情况明显低于野生型(WT),突变体的离体叶片在10 mmol/L H_2O_2处理下相比WT表现出更为明显的衰老症状。进一步遗传学分析表明,该突变体为单基因隐性突变。

关 键 词：Hydrogen peroxide hps12 Cotyledons greening rate CHLOROPHYLL SENESCENCE 

分 类 号：Q943.2[生物学—植物学]