Cloning and Bioinformatics Analysis of zmERECTA  

作　　者：Yihong JI Jinbao PAN Min LU Jun HAN Zhangjie NAN Qingpeng SUN 

机构地区：[1]Introduction Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture

出　　处：《Agricultural Science & Technology》2016年第4期819-821,共3页农业科学与技术（英文版）

基　　金：Supported by the Distinguished Young Scientists Project of Beijing(CIT&TCD201304096);Academic Degrees and Graduate Education Reform and Development Program of Beijing University of Agriculture(5056516002\016)

摘　　要：[Objective] This study was conducted to clone zmERECTA gene according to Arabidopsis ERECTA sequence and predict its characteristics by bioinformatics. [Method] The c DNA of zmERECTA gene was isolated from B73 using RT-PCR, and analyzed by bioinformatics methods. [Result] zmERECTA gene was 2 985 bp in size, which encoded a protein consisting of 944 amino acids, containing leucine-rich repeats, a PKC domain, two transmembrane regions, 14 N-glycosylation potential sites and41 kinase specific phosphorylation sites. The theoretical p I and molecular weight of zmERECTA protein was 6.01 and 10 8495.5respectively. [Conclusion] Cloning and bioinformatics of zmERECTA gene laid a foundation for further research.[Objective] This study was conducted to clone zmERECTA gene according to Arabidopsis ERECTA sequence and predict its characteristics by bioinformatics. [Method] The c DNA of zmERECTA gene was isolated from B73 using RT-PCR, and analyzed by bioinformatics methods. [Result] zmERECTA gene was 2 985 bp in size, which encoded a protein consisting of 944 amino acids, containing leucine-rich repeats, a PKC domain, two transmembrane regions, 14 N-glycosylation potential sites and41 kinase specific phosphorylation sites. The theoretical p I and molecular weight of zmERECTA protein was 6.01 and 10 8495.5respectively. [Conclusion] Cloning and bioinformatics of zmERECTA gene laid a foundation for further research.

关 键 词：Zea mays BIOINFORMATICS CLONE 

分 类 号：Q943.2[生物学—植物学]