黄秋葵多糖提取的响应面优化  

Optimization of Extraction of Polysaccharides from Okra by Response Surface Methodology

作　　者：颜玉华[1] 席雪纯 姚宏亮[1] 李宇东[1] 蔡广来 

机构地区：[1]金陵科技学院动物科学与技术学院,江苏南京210038

出　　处：《金陵科技学院学报》2016年第1期84-88,共5页Journal of Jinling Institute of Technology

基　　金：江苏省大学生创新创业训练计划指导项目(201513573051X)

摘　　要：通过响应面法对水浴提取黄秋葵中多糖的工艺条件进行优化。选定料液比、水浴时间和水浴温度作为影响因素,根据单因素试验的结果,利用三因素三水平Box-Behnken Design(BBD)设计法,得到关于黄秋葵多糖提取率的二次回归方程:Y=28.27+0.026A+0.014B-0.02C+0.095AB-2.5×10-3 AC+0.27BC-0.38A2-0.3B2-0.35C2。结果显示:最佳提取参数中液料比为63∶1(mL·g-1)、水浴时间为46min、水浴温度为77℃,多糖提取率达到最大值,为27.87%。Response surface methodology was applied to optimize the extraction of polysaccha- rides from okra by water bathing. Selected liquid/solid ratio, extraction time and extraction temperature were used as influence factors. According to the results of single-factor experi- ments, using 3 factors, 3 levels Box-Behnken Design, the established model was analyzed by BBD to obtain the optimum extraction conditions, and the regression equation was Y=28.27＋ 0. 026A ＋0. 014B- 0.02C ＋0. 095AB --2.5 × 10-3AC ＋0.27BC -0.38A2 -0.3B2 -0.35C2. The optimum parameters were： extraction time 46 min at 77 ℃ with ratio of liquid/solid 63：1 （mgog-1）, the yield of polysaccharide reached the maximum value of 27.87%.

关 键 词：黄秋葵 多糖 响应面法 

分 类 号：S649[农业科学—蔬菜学]