铈(Ⅲ)-刚果红-牛血清白蛋白体系共振线性和共振非线性散射光谱研究及应用  

Investigation and application of cerium(Ⅲ)-Congo red-bovine serum albumin system by resonance Rayleigh scattering and resonance non-linear scattering

作　　者：李国强[1,2] 何家洪[1,2] 徐强[1,2] 

机构地区：[1]重庆文理学院材料与化工学院,重庆402160 [2]环境材料与修复技术重庆市重点实验室(重庆文理学院),重庆402160

出　　处：《冶金分析》2016年第1期45-51,共7页Metallurgical Analysis

基　　金：重庆市教育委员会资助项目(KJ1401114);重庆市教育委员会资助项目(KJ131205);重庆文理学院重点项目(XSKY2014031);重庆文理学院重点项目(XSKY2015023)

摘　　要：基于铈一刚果红一牛血清白蛋白（Ce（Ⅲ）-CGR—BSA）三元离子缔合物的形成，构建了一种新的检测痕量铈的共振线性及共振非线性散射光谱（RNLS）分析法。研究发现，在PH7．6的NH3-NH4Cl缓冲溶液中，Ce（Ⅲ）能够与刚果红、牛血清白蛋白反应生成粒径更大、疏水性更强的三元离子缔合物，致使体系的共振瑞利散射（RRS）、二级散射（SOS）和倍频散射（FDS）强度迅速增加，其最大散射波长分别位于278、543和390nm处。通过条件试验确定以0．6mLpH7．6的NH3-NH4Cl缓冲溶液作为反应介质，0．025mg／mL刚果红溶液用量为1．8mL，0．1mg／mL牛血清白蛋白溶液用量为1．0mL，反应时间为5min，且大量存在的常见离子对Ce（Ⅲ）的测定不产生干扰。进一步考察发现，在各自最大散射波长处，3种散射光强度的增强（△IRRS，△ISOS和△IFDD）与Ce（Ⅲ）在2．0～10．0μg／mL范围内有良好的线性关系，对Ce（Ⅲ）的检出限（3d）分剐为0．29（RRS）、0．91（SOS）和3．54ng／mL（FDS）。同时，还对反应机理和散射光谱增强的原因进行了探讨。方法用于实际水样中铈的测定，相对标准偏差为0．94％～1．2％，测定值与原子吸收光谱法的结果相一致，加标回收率介于96％～102％之间。Based on the formation of ternary ion-association complex cerium（Ⅲ）-Congo red-bovine serum ablumin（Ce（Ⅲ）-CGR-BSA）, a new resonance Rayleigh scattering and resonance non-linear scattering meth- od has been developed for the determination of trace amounts of Ce（Ⅲ）. It was discovered that in NH3- NH4Cl buffer solution at pH 7.6, Ce（Ⅲ） could react with Congo red （CGR） and bovine serum albumin （BSA） to form larger size and more hydrophobic ternary ion-association, which caused the resonance Ray- leigh scattering （RRS）, second-order scattering （SOS） and frequency double scattering （FDS） intensities dramatically enhanced. The maximum scattering wavelength of RRS, SOS and FDS were located at 278, 543, 390 nm, respectively. Through condition test, it was determined that 0.6 mL of NH3-NH4Cl buffer solution at pH 7. 6 was used as the reaction medium, 1.8 mL of 0. 025 mg/mL CGR solution and 1.0 mL of 0.1 mg/mL BSA solution was used. The reaction time was 5 min, and the presence of a large amount of common coexisting ions had no interference with the determination of Ce（Ⅲ）. Further investigation showed that the enhancement of scattering intensities （△IRRS, △ISOS and △IFDS） was linear to the Ce（Ⅲ） mass concen- tration in the range of 2.0-10.0 μg/mL, and the detection limits （3a） were 0.29 ng/mL for RRS, 0.91 ng/ mL for SOS and 3.54 ng/mL for FDS. Moreover, the reaction mechanism and the reason for scattering en- hancement were also discussed. The proposed method was applied to the determination of Ce（Ⅲ） in actual water sample, the relative standard deviation was 0. 94%-1. 2%. The found results were consistent with those obtained by atomic absorption spectrometry（AAS）, the recovery was between 96% and 102%.

关 键 词：铈 刚果红 牛血清白蛋白 共振瑞利散射 二级散射 倍频散射 

分 类 号：O657.3[理学—分析化学] O614.332[理学—化学]