Box-Behnken响应面法结合多指标综合加权法优选升麻中酚酸类成分的提取工艺  

Optimization of the Extraction Technology of Phendic Acids in Cimicifugae foetidae by Using Box-Behnken Response Surface Methodology Combined with Multi-index Comprehensive Weighted Method

作　　者：范孟雪[1,2,3] 秦昆明[1,2,3] 丁斐 黄雨婷[1,2,3] 蔡宝昌[1,2,3] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,南京210046 [2]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,南京210023 [3]南京海昌中药集团有限公司/江苏省企业研究生工作站,南京210061

出　　处：《中国药房》2016年第13期1835-1838,共4页China Pharmacy

基　　金：江苏省科技项目(No.BK20141093)

摘　　要：目的:优化升麻中酚酸类成分的提取工艺。方法:在单因素试验的基础上,以乙醇体积分数、回流提取时间和液料比3个因素为自变量,以咖啡酸、阿魏酸、异阿魏酸含量和浸出物得率的综合评分为因变量,采用基于Box-Behnken设计的响应面法优化升麻中酚酸类成分的提取工艺。结果:最优提取工艺为以8倍药材量的70%乙醇提取2次,每次200 min。验证试验结果表明,综合评分指标的预测值与实测值相对偏差仅为2.20%。结论:优选的提取工艺方法简单、稳定可行,可为后续升麻药材的制剂生产提供参考。OBJECTIVE：To optimize the extraction technology of phenolic acids from Cimicifugae foetidae. METHODS：Based on single factor test,the extraction technology of phenolic acids from C. foetidae was optimized by response surface methodology based on Box-Behnken design using ethanol volume fraction,reflux extraction time and liquid to solid ratio as independent variables,comprehensive score of the content of caffeic acid,ferulic acid and isoferulic and extract yield as dependent variable. RESULTS：The optimal extraction technology was as follows as 8-fold 70% ethanol,extracting for 2 times,200 min each time. Results of validation test indicated that the difference between predicted value of comprehensive score indicators and measured value was 2.20%. CONCLUSIONS：The optimal extraction technology is simple,stable and feasible,and can provide reference for following production of C. foetidae preparation.

关 键 词：升麻 咖啡酸 阿魏酸 异阿魏酸 含量 浸出物得率 BOX-BEHNKEN设计 响应面法 提取工艺 

分 类 号：R983[医药卫生—药品]